Cell-material interactions on biphasic polyurethane matrix.
Cell-material interactions on biphasic polyurethane matrix.
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DOI：
10.1002/jbm.a.34515
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发表时间：
2013-08
影响因子：
4.9
通讯作者：
Sarkar, Debanjan
中科院分区：
文献类型：
--
作者：
Dicesare, Patrick;Fox, Wade M.;Hill, Michael J.;Krishnan, G. Rajesh;Yang, Shuying;Sarkar, Debanjan
细胞-基质相互作用是再生组织工程中控制干细胞命运的关键调节因子。这些相互作用由细胞外基质的纳米级特征诱导和控制，并在合成基质上模拟以控制细胞结构和功能。最近的研究表明，纳米结构基质可以调节干细胞的行为，并在组织再生中发挥特定的作用。在这项研究中，我们已经证明，合成基质的纳米结构相形态可以控制人间充质干细胞（MSC）的粘附，增殖，组织和迁移。具有链段组成的纳米结构的生物可降解聚氨酯（PU）在纳米尺度上表现出双相形态，并且可以控制MSC的细胞特征。设计了由脂肪族二异氰酸酯和二肽扩链剂组成的聚酯软段和硬段的生物可降解聚氨酯，考察了其对聚氨酯相形态的影响。通过改变聚氨酯的组成，PU的形态结构进行调制，并在MSC上检查其效果。结果表明，骨髓间充质干细胞可以感觉到双相聚氨酯基质的纳米级形态，表现出独特的细胞特征，因此，标志着基质相形态的相关性。合成基质的纳米结构相在控制细胞-基质相互作用中的作用为合成基质上细胞行为的调节提供了重要的见解，因此是工程组织再生的重要工具。
Cell–matrix interaction is a key regulator for controlling stem cell fate in regenerative tissue engineering. These interactions are induced and controlled by the nanoscale features of extracellular matrix and are mimicked on synthetic matrices to control cell structure and functions. Recent studies have shown that nanostructured matrices can modulate stem cell behavior and exert specific role in tissue regeneration. In this study, we have demonstrated that nanostructured phase morphology of synthetic matrix can control adhesion, proliferation, organization and migration of human mesenchymal stem cells (MSCs). Nanostructured biodegradable polyurethanes (PU) with segmental composition exhibit biphasic morphology at nanoscale dimensions and can control cellular features of MSCs. Biodegradable PU with polyester soft segment and hard segment composed of aliphatic diisocyanates and dipeptide chain extender were designed to examine the effect polyurethane phase morphology. By altering the polyurethane composition, morphological architecture of PU was modulated and its effect was examined on MSC. Results show that MSCs can sense the nanoscale morphology of biphasic polyurethane matrix to exhibit distinct cellular features and, thus, signifies the relevance of matrix phase morphology. The role of nanostructured phases of a synthetic matrix in controlling cell–matrix interaction provides important insights for regulation of cell behavior on synthetic matrix and, therefore, is an important tool for engineering tissue regeneration.
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影响因子：
2.9
作者：
Arnold, M;Cavalcanti-Adam, EA;Spatz, JP
通讯作者：
Spatz, JP
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
14
作者：
Li, Bing;Yoshii, Toshitaka;Guelcher, Scott A.
通讯作者：
Guelcher, Scott A.
影响因子：
5.5
作者：
ESTES, GM;SEYMOUR, RW;COOPER, SL
通讯作者：
COOPER, SL
影响因子：
5.5
作者：
Hernandez, Rebeca;Weksler, Jadwiga;Runt, James
通讯作者：
Runt, James