IMMUNOHISTOCHEMICAL AND ELECTRON-MICROSCOPIC STUDY OF INTERACTION OF YERSINIA-ENTEROCOLITICA SEROTYPE-O8 WITH INTESTINAL-MUCOSA DURING EXPERIMENTAL ENTERITIS
IMMUNOHISTOCHEMICAL AND ELECTRON-MICROSCOPIC STUDY OF INTERACTION OF YERSINIA-ENTEROCOLITICA SEROTYPE-O8 WITH INTESTINAL-MUCOSA DURING EXPERIMENTAL ENTERITIS
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DOI：
10.1128/iai.57.3.673-678.1989
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发表时间：
1989-03-01
影响因子：
3.1
通讯作者：
RIECKEN, EO
中科院分区：
文献类型：
--
作者：
HANSKI, C;KUTSCHKA, U;RIECKEN, EO
对小鼠实验性感染O8型耶尔森氏菌进行了定量和组织学研究。通过免疫组织化学染色准确确定细菌的侵袭和扩散过程。口服后，细菌穿过回肠上皮屏障，扩散到固有层。他们优先进入Peyer的S斑块，后者的定植密度大约是周围同等表面积上皮细胞的1,000倍。细菌在毛囊中增殖，从毛囊扩散到绒毛的固有层。在这两个部位，大多数细菌都在细胞外繁殖，只有一小部分存在于吞噬细胞内。细菌似乎无法通过完整的基底膜；因此，基底膜的完整性可能在决定小肠结肠炎耶尔氏杆菌感染的进入途径和传播限制方面发挥作用。
The experimental infection of mice with Yersinia enterocolitica serotype O8 was investigated in a quantitative and histological study. The course of bacterial penetration and spreading was precisely determined by immunohistochemical staining. After oral administration, the bacteria passed the epithelial barrier of the ileum and spread into the lamina propria. By preference they entered Peyer''s patches, which were about 1,000 times more heavily colonized than the surrounding epithelium of a comparable surface area. The bacteria proliferated in the follicles, from which they spread into the lamina propria of the villi. At either site most of the bacteria multiplied extracellularly, with only a small percentage observed to be present within the phagocytes. The bacteria did not appear to be able to pass the intact basement membrane; hence, the integrity of the basement membrane is likely to play a role in determining the route of entry and limit of spread of Y. enterocolitica infection.