Enhanced chemosensitivity of chick parasympathetic neurones in co-culture with myotubes.
Enhanced chemosensitivity of chick parasympathetic neurones in co-culture with myotubes.
复制标题
与肌管共培养的鸡副交感神经元的化学敏感性增强。
DOI：
10.1113/jphysiol.1982.sp014366
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Vaca,K
中科院分区：
文献类型：
--
作者：
Crean,G;Pilar,G;Tuttle,JB;Vaca,K
1.通过测定神经元胞体膜对乙酰胆碱（ACh）的敏感性，研究了靶点相互作用对睫状神经节细胞电生理特性的影响。随着培养时间的推移，测量了对发射器有反应的细胞百分比的变化。2.接种后24小时，所有细胞对ACh的离子电泳脉冲产生去极化反应。然而，接种后1周（7 - 14天），大多数神经元无反应，高度反应细胞（大于100 mV峰值去极化/nC）极为罕见。甚至在培养的后期，对递质敏感的神经元再次变得更加频繁。3.然而，当将神经元接种到预先形成的胸肌肌管上时，ACh敏感性在整个3周的培养期内保持不变。神经肌肉接头由神经元形成，当存在足够的神经元时，所有测试的肌纤维都显示出功能性突触传递的证据。对ACh的化学敏感性不是由无肌肉微培养物中的神经元维持的，它们存在于同一盖玻片上。4.由超微结构标准定义的神经元间突触接触在单独的神经元培养物中形成，但在培养7 - 14天时未发现细胞之间广泛功能性突触相互作用的证据。5.结论是，培养的睫状神经节细胞的ACh敏感性的维持通过共培养中肌肉的存在而增强。在这些培养条件下，观察到的神经元间突触接触显然不如与肌肉共培养对胆碱能表型的完全表达那样有效。
1. The influence of target interaction upon the electrophysiological properties of dissociated ciliary ganglion cells was investigated by testing the sensitivity of the neuronal somal membrane to ionophoretically applied acetylcholine (ACh). Variations in the percentage of cells responsive to the transmitter were measured with time in culture. 2. Twenty‐four hours after plating, all cells respond to an ionophoretic pulse of ACh with a depolarization. However, 1 week after plating (between 7 and 14 days) most of the neurones are unresponsive, and highly responsive cells (greater than 100 mV peak depolarization/nC) are extremely rare. At even later times in culture, neurones sensitive to the transmitter are again more frequent. 3. When neurones are plated onto pre‐formed pectoral myotubes, however, ACh sensitivity is maintained throughout a 3 week culture period. Neuromuscular junctions are formed by the neurones, and when sufficient neurones are present, all the muscle fibres tested show evidence of functional synaptic transmission. Chemosensitivity to ACh is not maintained by neurones in muscle‐free microcultures are present on the same cover‐slip. 4. Interneuronal synaptic contacts, defined by ultrastructural criteria, are formed in cultures of neurones alone, but evidence of widespread functional synaptic interaction between cells was not found at 7‐14 days in culture. 5. It is concluded that the maintenance of ACh sensitivity of cultured ciliary ganglion cells is enhanced by the presence of muscle in co‐culture. The interneuronal synaptic contacts observed are apparently not as potent a stimulus as co‐culture with muscle for the full expression of the cholinergic phenotype under these culture conditions.