Green-Synthesization of Silver Nanoparticles Using Endophytic Bacteria Isolated from Garlic and Its Antifungal Activity against Wheat Fusarium Head Blight Pathogen Fusarium graminearum
Green-Synthesization of Silver Nanoparticles Using Endophytic Bacteria Isolated from Garlic and Its Antifungal Activity against Wheat Fusarium Head Blight Pathogen Fusarium graminearum
复制标题
大蒜内生细菌绿色合成银纳米粒子及其对小麦赤霉病菌禾谷镰刀菌的抗真菌活性
DOI：
10.3390/nano10020219
复制
发表时间：
2020-02-01
期刊：
影响因子：
5.3
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Ibrahim, Ezzeldin;Zhang, Muchen;Li, Bin
纳米颗粒有望在未来的植物病害管理中发挥至关重要的作用，并有望为传统的合成杀菌剂提供一种环境友好的替代品。本研究以大蒜（Allium sativum）中分离的内生细菌Poae假单胞菌CO为载体，通过介导法合成了银纳米颗粒（AgNPs），并对其进行了绿色的表征。采用UV-Vis进行确认分析后，我们检测了粒径为19.8-44.9 nm的生物合成AgNPs的体外抗真菌活性，其对小麦赤霉病病原菌Fusarium graminearum的菌丝生长、孢子萌发、芽管长度和真菌毒素产生均表现出较强的抑制作用。此外，显微镜检查表明，菌丝体的形态有畸形和崩溃时，用AgNPs处理，导致DNA和蛋白质泄漏到细胞外。利用X射线衍射、透射电镜、扫描电镜、能谱仪和傅里叶变换红外光谱等对生物合成的具有较强抗真菌活性的银纳米粒子进行了表征。总体而言，本研究的结果清楚地表明，生物合成的AgNPs在保护小麦免受真菌感染方面可能具有很大的潜力。
Nanoparticles are expected to play a vital role in the management of future plant diseases, and they are expected to provide an environmentally friendly alternative to traditional synthetic fungicides. In the present study, silver nanoparticles (AgNPs) were green synthesized through the mediation by using the endophytic bacterium Pseudomonas poae strain CO, which was isolated from garlic plants (Allium sativum). Following a confirmation analysis that used UV-Vis, we examined the in vitro antifungal activity of the biosynthesized AgNPs with the size of 19.8-44.9 nm, which showed strong inhibition in the mycelium growth, spore germination, the length of the germ tubes, and the mycotoxin production of the wheat Fusarium head blight pathogen Fusarium graminearum. Furthermore, the microscopic examination showed that the morphological of mycelia had deformities and collapsed when treated with AgNPs, causing DNA and proteins to leak outside cells. The biosynthesized AgNPs with strong antifungal activity were further characterized based on analyses of X-ray diffraction, transmission electron microscopy, scanning electron microscopy, EDS profiles, and Fourier transform infrared spectroscopy. Overall, the results from this study clearly indicate that the biosynthesized AgNPs may have a great potential in protecting wheat from fungal infection.