Circular RNA has_circ_0000527 participates in proliferation, invasion and migration of retinoblastoma cells via miR-646/BCL-2 axis
Circular RNA has_circ_0000527 participates in proliferation, invasion and migration of retinoblastoma cells via miR-646/BCL-2 axis
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DOI：
10.1002/cbf.3535
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发表时间：
2020-04-07
影响因子：
3.6
通讯作者：
Li, Xiu-gui
中科院分区：
文献类型：
--
作者：
Chen, Nan-nan;Chao, Dai-ling;Li, Xiu-gui
视网膜母细胞瘤是儿童最常见的原发性眼内恶性肿瘤之一。新兴的研究表明，环状RNA（circRNA）在各种癌症中起着关键作用。作为一种新的circRNA，circ_0000527在RB中的功能尚不清楚。本研究采用实时荧光定量聚合酶链反应（qRT-PCR）检测RB组织和细胞系中circ_0000527和miR-646的表达水平。RB细胞系（SO-Rb 50和WERI-Rb-1）用作功能实验中的细胞模型。采用CCK-8法、TUNEL法和transwell法检测circ_0000527和miR-646对肿瘤细胞的体外生物学效应。采用qRT-PCR、荧光素酶报告基因分析、RIP分析和western blot检测circ_0000527、miR-646和BCL-2之间的相互作用。结果表明，与正常组织相比，RB样品中circ_0000527的表达水平显著上调，而miR-646的表达水平显著下调。过表达circ_0000527可促进RB细胞的存活、迁移和侵袭，而转染miR-646则相反。Circ_0000527吸收miR-646以调节BCL-2的表达。结论：circ_0000527可能通过吸收miR-646间接调节BCL-2，从而促进RB的发生发展。研究意义：与正常组织相比，circ_0000527的表达水平显著上调，而miR-646的表达水平显著下调。过表达circ_0000527可促进RB细胞的存活、迁移和侵袭，而转染miR-646则相反。Circ_0000527吸收miR-646以调节BCL-2的表达。
Retinoblastoma (RB) is one of the most common primary intraocular malignancies in children. Emerging researches have shown that circular RNAs (circRNAs) play critical roles in a variety of cancers. As a novel circRNA, the function of circ_0000527 in RB remains unknown. In this work, expression level of circ_0000527 and miR-646 in RB tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). RB cell lines (SO-Rb50 and WERI-Rb-1) were used as cell models in functional experiments. CCK-8 assay, TUNEL assay and transwell assay were employed to detect the biological influence of circ_0000527 and miR-646 on cancer cells in vitro. qRT-PCR, luciferase reporter assay, RIP assay and western blot were used for exploring the interactions among circ_0000527, miR-646 and BCL-2. It was demonstrated that expression level of circ_0000527 in RB samples was significantly up-regulated compared to normal tissues, while miR-646 was markedly down-regulated. Overexpression of circ_0000527 promoted the viability, migration and invasion of RB cells, while miR-646 transfection had the opposite effects. Circ_0000527 sponged miR-646 to regulate the expression of BCL-2. In conclusion, circ_0000527 could promote the development of RB by indirectly modulating BCL-2 via absorbing miR-646.Significance of the study Expression level of circ_0000527 in RB samples was significantly up-regulated compared to normal tissues, while miR-646 was markedly down-regulated. Overexpression of circ_0000527 promoted the viability, migration and invasion of RB cells, while miR-646 transfection had the opposite effects. Circ_0000527 sponged miR-646 to regulate the expression of BCL-2.