A common set of developmental miRNAs are upregulated in Nicotiana benthamiana by diverse begomoviruses.
A common set of developmental miRNAs are upregulated in Nicotiana benthamiana by diverse begomoviruses.
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DOI：
10.1186/1743-422x-8-143
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发表时间：
2011-03-29
期刊：
影响因子：
4.8
通讯作者：
Fauquet CM
中科院分区：
文献类型：
--
作者：
Amin I;Patil BL;Briddon RW;Mansoor S;Fauquet CM
BegomoVirus是一种单链DNA病毒，可引起世界各地许多农作物的重要经济疾病，并在植物上引起类似发育异常的症状，包括端粒、卷叶和发育迟缓。MicroRNAs(MiRNAs)是一类小的内源RNA，参与植物发育、信号转导、蛋白质降解以及对环境胁迫和病原体入侵的反应等多种活动。本研究旨在了解miRNAs对巨细胞病毒感染的解除调控。利用非洲木薯花叶病毒(ACMV)、卷心菜曲叶病毒(CbLCuV)、番茄黄化曲叶病毒(TYLCV)和棉花曲叶木马病毒/棉花曲叶卫星病毒(CLCuV/CLCuMB)4种不同的病毒类型进行了研究。对10个发育中的miRNA进行了研究。将该病毒接种到本氏拟青霉植株上，用特异的miRNA探针进行Northern杂交，分析其miRNA图谱。TYLCV、CLCuMV/CLCuMB和CbLCuV虽然基因组组成不同，但大多数发育过程中的miRNA水平均升高。然而，不同的秋病毒个体miRNAs水平的增加是不同的，反映了症状表型的严重程度的差异。其中一些miRNA在ACMV感染中也是常见的。我们的结果显示了BegomVirus感染时miRNAs积累的共同模式。研究发现，秋病毒一般会增加miRNA的积累，从而导致参与植物发育的基因翻译减少。识别在巨细胞病毒感染时被解除调控的常见miRNAs可能为旨在发展广谱抗药性的控制策略提供新的靶点。
Begomoviruses are single-stranded DNA viruses that cause economically important diseases of many crops throughout the world and induce symptoms in plants, including enations, leaf curling and stunting, that resemble developmental abnormalities. MicroRNAs (miRNAs) are small endogenous RNAs that are involved in a variety of activities, including plant development, signal transduction and protein degradation, as well as response to environmental stress, and pathogen invasion. The present study was aimed at understanding the deregulation of miRNAs upon begomovirus infection. Four distinct begomoviruses African cassava mosaic virus (ACMV), Cabbage leaf curl virus (CbLCuV), Tomato yellow leaf curl virus (TYLCV) and Cotton leaf curl Multan virus/Cotton leaf curl betasatellite (CLCuV/CLCuMB), were used in this study. Ten developmental miRNA were studied. N. benthamiana plants were inoculated with begomoviruses and their miRNA profiles were analysed by northern blotting using specific miRNA probes. The levels of most developmental miRNA were increased in N. benthamiana by TYLCV, CLCuMV/CLCuMB and CbLCuV infection with a common pattern despite their diverse genomic components. However, the increased levels of individual miRNAs differed for distinct begomoviruses, reflecting differences in severity of symptom phenotypes. Some of these miRNA were also common to ACMV infection. Our results have shown a common pattern of miRNAs accumulation upon begomovirus infection. It was found that begomoviruses generally increase the accumulation of miRNA and thus result in the decreased translation of genes involved in the development of plants. Identification of common miRNAs that are deregulated upon begomovirus infection may provide novel targets for control strategies aimed at developing broad-spectrum resistance.