Proviral loads of human T-lymphotropic virus Type 1 in asymptomatic carriers with different infection routes
Proviral loads of human T-lymphotropic virus Type 1 in asymptomatic carriers with different infection routes
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DOI：
10.1002/ijc.26289
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发表时间：
2012-05-15
影响因子：
6.4
通讯作者：
Okayama, Akihiko
中科院分区：
文献类型：
--
作者：
Ueno, Shiro;Umeki, Kazumi;Okayama, Akihiko
高人类T淋巴细胞病毒1型（HTLV-1）前病毒DNA载量（PVL）已被报道是成人T细胞白血病/淋巴瘤（ATL）发展的一个危险因素。ATL也被认为在围产期获得感染的HTLV-1携带者中发展。据报道，ATL细胞经常携带有缺陷的前病毒。在我们的研究中，检测了309名不同感染途径的无症状携带者的HTLV-1前病毒3个不同区域（5 'LTR-gag、gag和pX）的PVL。PVL的pX区域在21无症状携带者与母亲感染显着高于24携带者与配偶感染。在161名具有相对高pX PVL（等于或大于1拷贝/100个外周血单核细胞）的携带者中，26名携带者（16%）具有低gag PVL/pX PVL（小于0.5），4名携带者（2%）具有低5 'LTR-gag PVL/pX PVL（小于0.5）。低gag PVL/pX PVL比值反映了HTLV-1前病毒gag区DNA序列的缺陷和/或多态性，也与母体感染有关。这些数据表明，HTLV-1携带者的母亲感染往往有高PVL，这可能与前病毒的缺陷和/或前病毒DNA序列的多态性。此外，该比率有可能用作区分无症状HTLV-1携带者感染途径的工具，这支持了大规模研究的需要。
High human T-lymphotropic virus Type 1 (HTLV-1) proviral DNA load (PVL) has been reported to be one risk factor for the development of adult T-cell leukemia/lymphoma (ATL). ATL is also believed to develop in HTLV-1 carriers who acquire infection perinatally. ATL cells have been reported to frequently harbor defective provirus. In our study, PVLs for three different regions of HTLV-1 provirus (5'LTR-gag, gag and pX) were measured in 309 asymptomatic carriers with different infection routes. PVLs for the pX region in 21 asymptomatic carriers with maternal infection was significantly higher than in 24 carriers with spousal infection. Among 161 carriers with relatively high pX PVLs (equal to or greater than 1 copy per 100 peripheral blood mononuclear cells), 26 carriers (16%) had low gag PVL/pX PVL (less than 0.5) and four (2%) had low 5'LTR-gag PVL/pX PVL (less than 0.5). Low gag PVL/pX PVL ratio, which reflects deficiency and/or polymorphism of HTLV-1 proviral DNA sequences for the gag region, was also associated with maternal infection. These data suggest that HTLV-1 carriers with maternal infection tend to have high PVLs, which may be related to provirus with deficiency and/or the polymorphism of proviral DNA sequences. In addition, there is a possibility that this ratio may be used as a tool to differentiate the infection routes of asymptomatic HTLV-1 carriers, which supports the need for a large scale study.