CP4: a pneumocyte-derived collagenous surfactant-associated protein. Evidence for heterogeneity of collagenous surfactant proteins.
CP4: a pneumocyte-derived collagenous surfactant-associated protein. Evidence for heterogeneity of collagenous surfactant proteins.
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CP4：一种肺细胞来源的胶原表面活性剂相关蛋白。
DOI：
10.1021/bi00423a011
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Crouch,E
中科院分区：
文献类型：
--
作者：
Persson,A;Rust,K;Chang,D;Moxley,M;Longmore,W;Crouch,E
材料和方法II型细胞分离。大鼠II型细胞通过Dobbs等人的方法的有限修改来分离。（1986年）。简而言之，将成年雄性Sprague-Dawley大鼠麻醉、肝素化、气管造口，并如前所述通过放血处死（Crouch等人，1987年a）。通过肺动脉用磷酸盐缓冲盐水（PBS）灌注肺以去除血液，并通过气管用二价无阳离子HEPES缓冲盐水反复灌洗以获得粗表面活性剂并去除巨噬细胞。猪胰腺弹性蛋白酶（Elastin Products，Inc.，Pacific，MO; 30地衣红单位/mL）在37 ℃下以三个等分试样滴入气道中，每个等分试样达到总肺容量，总共20分钟。在250 ng/mL DNA酶I（Boehringer-Mannheim）的存在下切碎肺，并通过酶灭活弹性蛋白酶。
Materials and MethodsType IICell Isolation. Rat type II cells were isolated by limited modifications of the method of Dobbs et al.(1986). Briefly, adult male Sprague-Dawley rats were anesthetized, heparinized, tracheostomized, and sacrificed by exsanguination as previously described (Crouch et al., 1987a). The lungs were perfused via the pulmonaryartery with phosphate-buffered saline (PBS) to remove blood and lavaged repeatedly via the trachea with divalent cation-free HEPES-buffered saline to obtain crude surfactant and to remove macrophages. Porcine pancreatic elastase (Elastin Products, Inc., Pacific, MO; 30 orcein units/mL) was instilled into the airway in three aliquots, each to total lung capacity, for a total of 20 min at 37 C. The lungs were minced in the presence of 250/ng/mL DNase I (Boehringer-Mannheim), and elastase was inactivated by the