Community level physiological profiles of bacterial communities inhabiting uranium mining impacted sites
Community level physiological profiles of bacterial communities inhabiting uranium mining impacted sites
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DOI：
10.1016/j.ecoenv.2013.11.012
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发表时间：
2014-02-01
影响因子：
6.8
通讯作者：
Boteva, Silvena
中科院分区：
文献类型：
--
作者：
Kenarova, Anelia;Radeva, Galina;Boteva, Silvena
Bacterial activity and physiological diversity were characterized in mining and milling impacted soils collected from three abandoned uranium mine sites, Senokos, Buhovo and Sliven, using bacterial dehydrogenase activity and Biolog (EcoPlate) tests. The elemental composition of soils revealed high levels of uranium and heavy metals (sum of technogenic coefficients of contamination; TCCsum) pollution as follows: Sliven (uranium - 374 mg/kg; TCCsum - 23.40) > Buhovo (uranium - 139.20 mg/kg; TCCsum - 3.93) > Senokos (uranium - 23.01 mg/kg; TCCsum - 0.86). The physiological profiles of the bacterial community level were site specific, and indicated intensive utilization of polyols, carbohydrates and carboxylic acids in low and medium polluted environments, and i-erithrytol and 2-hydroxy-benzoic acid in the highly polluted environment of Sliven waste pile. Enzymes which take part in the biodegradation of recalcitrant substances were more resistant to pollution than these from the pathways of the easily degradable carbon sources. The Shannon index indicated that the physiological diversity of bacteria was site specific but not in line with the levels of pollution. A general tendency of increasing the importance of the number of utilizable substrates to bacterial physiological diversity was observed at less polluted sites, whereas in highly polluted sites the evenness of substrate utilization rate was more significant. Dehydrogenase activity was highest in Senokos upper soil layer and positively correlated (p
