Establishment of IPC 227 cells as human xenografts in rabbits: a model of uveal melanoma
Establishment of IPC 227 cells as human xenografts in rabbits: a model of uveal melanoma
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DOI：
10.1097/00008390-200010000-00006
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发表时间：
2000-10-01
影响因子：
2.2
通讯作者：
Madelmont, JC
中科院分区：
文献类型：
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作者：
Bonicel, P;Michelot, J;Madelmont, JC
本研究旨在探讨建立人葡萄膜黑色素瘤动物模型的可行性。IPC 227是从梭形细胞睫状体黑色素瘤患者的活检中建立的细胞系，将其移植到免疫抑制的新西兰兔的眼前房中。在第二步中，将来自前房的肿瘤碎片经巩膜植入后脉络膜中，然后对动物进行完整的眼科检查。根据植入位置，观察到特征性生长模式。在前房中，在注射细胞悬液后8天出现了弥漫性、平坦的、色素沉着的肿瘤，该细胞悬液覆盖虹膜和房角，到第25天，成功率为100%。在脉络膜下植入后6-7周获得结节状、浅色素沉着的肿瘤，成功率为25%。临床检查，包括眼底照相，超声和磁共振成像，表现出与人葡萄膜黑色素瘤相同的特征，证实了该模型在评估葡萄膜黑色素瘤新的治疗和诊断方法中的价值。(C)2000年利平科特威廉姆斯&威尔金斯。
This study was designed in order to evaluate the feasibility of establishing an animal model of human uveal melanoma. IPC227, a cell line established from the biopsy of a patient with a spindle cell ciliary body melanoma, was transplanted into the anterior chamber of the eye in immunosuppressed New Zealand rabbits. In a second step, a tumour fragment from the anterior chamber was implanted transclerally into the posterior choroid, Complete ophthalmological examinations were then performed on the animals. Characteristic growth patterns were noted depending on the location of implantation. In the anterior chamber, diffuse, flat, heavily pigmented tumours appeared 8 days after the injection of the cell suspension that covered the iris and the angle by day 25, with a success rate of 100%. Nodular, lightly pigmented tumours were obtained 6-7 weeks after subchoroidal implantation, with a 25% success rate. Clinical examination, including fundus photography, ultrasound and magnetic resonance imaging, demonstrated the same characteristics as those of human uveal melanoma, confirming the value of this model for the evaluation of new therapeutic and diagnostic methods in the management of uveal melanoma. (C) 2000 Lippincott Williams & Wilkins.