FLUORESCENCE DEPOLARIZATION STUDIES OF PHASE-TRANSITIONS AND FLUIDITY IN PHOSPHOLIPID BILAYERS .1. SINGLE COMPONENT PHOSPHATIDYLCHOLINE LIPOSOMES
FLUORESCENCE DEPOLARIZATION STUDIES OF PHASE-TRANSITIONS AND FLUIDITY IN PHOSPHOLIPID BILAYERS .1. SINGLE COMPONENT PHOSPHATIDYLCHOLINE LIPOSOMES
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DOI：
10.1021/bi00665a029
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
THOMPSON, TE
中科院分区：
文献类型：
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作者：
LENTZ, BR;BARENHOLZ, Y;THOMPSON, TE
利用疏水性荧光探针1，6-二苯基-1，3，5-己三烯的荧光去偏振来监测合成磷脂酰胆碱分散体中凝胶-液晶相变过程中流动性的变化。测定了大的多层脂质体和小的单层囊泡的相变参数。将这些参数与使用其他技术获得的参数进行了比较。这些数据根据2个极限分子模型进行解释，这些模型反过来又提供了对多层脂质体和小囊泡之间的结构差异的洞察。
The fluorescence depolarization associated with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene is used to monitor the changes in fluidity accompanying the gel-liquid crystalline phase transition in synthetic phosphatidylcholine dispersions. The parameters of the phase transition are determined for both large, multilamellar liposomes and small, single-lamellar vesicles. These parameters are compared with those obtained using other techniques. The data are interpreted in terms of 2 limiting molecular models, which in turn offer insight into the structural differences between multilamellar liposomes and small vesicles.