Early-life adversity accelerates cellular ageing and affects adult inflammation: Experimental evidence from the European starling.
Early-life adversity accelerates cellular ageing and affects adult inflammation: Experimental evidence from the European starling.
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DOI：
10.1038/srep40794
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发表时间：
2017-01-17
影响因子：
4.6
通讯作者：
Bateson M
中科院分区：
文献类型：
--
作者：
Nettle D;Andrews C;Reichert S;Bedford T;Kolenda C;Parker C;Martin-Ruiz C;Monaghan P;Bateson M
早期生活逆境与发育期间细胞加速老化和成年期炎症增加有关。然而，人类研究只能建立相关性，而不是因果关系，现有的实验动物方法同时改变了早期生活逆境的多个组成部分。我们开发了一种新的手工饲养模式，在欧洲椋鸟雏鸟（Sturnus vulgaris），在其中我们分别操纵营养不足和乞讨的努力，为期10天。实验处理加速了红细胞端粒的磨损，并增加了在青少年时期测量的DNA损伤。对于端粒磨损，食物量和乞讨努力产生加性效应。只有低食物量和高乞讨努力的结合才能增加DNA损伤。然后，我们测量了两个炎症标志物，高敏感性C反应蛋白和白细胞介素-6，当鸟是成年人。实验性治疗影响了两种炎症标志物，尽管每种标志物的模式都很复杂且不同。实验处理对成人白细胞介素-6的影响部分是通过增加青少年DNA损伤介导的。我们的研究结果表明，营养输入和乞讨努力在雏鸟期影响细胞衰老和成年炎症的八哥。然而，在不同时间点测量的不同生物标志物的效应模式是不同的。
Early-life adversity is associated with accelerated cellular ageing during development and increased inflammation during adulthood. However, human studies can only establish correlation, not causation, and existing experimental animal approaches alter multiple components of early-life adversity simultaneously. We developed a novel hand-rearing paradigm in European starling nestlings (Sturnus vulgaris), in which we separately manipulated nutritional shortfall and begging effort for a period of 10 days. The experimental treatments accelerated erythrocyte telomere attrition and increased DNA damage measured in the juvenile period. For telomere attrition, amount of food and begging effort exerted additive effects. Only the combination of low food amount and high begging effort increased DNA damage. We then measured two markers of inflammation, high-sensitivity C-reactive protein and interleukin-6, when the birds were adults. The experimental treatments affected both inflammatory markers, though the patterns were complex and different for each marker. The effect of the experimental treatments on adult interleukin-6 was partially mediated by increased juvenile DNA damage. Our results show that both nutritional input and begging effort in the nestling period affect cellular ageing and adult inflammation in the starling. However, the pattern of effects is different for different biomarkers measured at different time points.