Cytochrome P450 1A1, glutathione S-transferases M1 and T1 polymorphisms in Ovambos and Mongolians.
Cytochrome P450 1A1, glutathione S-transferases M1 and T1 polymorphisms in Ovambos and Mongolians.
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DOI：
10.1016/j.legalmed.2009.01.073
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发表时间：
2009-04-01
期刊：
影响因子：
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通讯作者：
Takeshita, Haruo
中科院分区：
文献类型：
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作者：
Fujihara, Junko;Yasuda, Toshihiro;Takeshita, Haruo
细胞色素P450（GST）1A 1、谷胱甘肽S-转移酶（GST）M1和GSTT 1基因多态性已被证明与几种疾病相关。本研究对134名蒙古族和207名蒙古族人群的CYP 1A 1 MspI、GSTM 1和GSTT 1基因多态性进行了研究，并与前人的研究结果进行了比较。采用聚合酶链反应/限制性片段长度多态性（PCR-RFLP）的频率CYP 1A 1 MspI突变进行了测定。采用多重PCR方法检测GSTM 1和GSTT 1基因多态性。蒙古族和鄂温克族CYP 1A 1 MspI基因型的野生型、杂合型和纯合型频率分别为22.7%、55.6%和21.7%。蒙古族和蒙古族GSTM 1（null）基因型频率分别为46.4%和25.6%。Ovambos的CYP 1A 1 MspI和GSTT 1（null）基因型分布与非洲裔美国人和一些高加索人相似。相反，GSTM 1（空）基因型分布与所有其他人群不同。在蒙古族人群中，CYP 1A 1 MspI多态性的突变频率最高，GSTM 1（null）与东亚人群相似，GSTT 1（null）与几乎所有的亚洲人群不同。
Cytochrome P450 (CYP) 1A1, glutathione S-transferase (GST) M1, and GSTT1 gene polymorphisms have been shown to be associated with several diseases. In this study, CYP1A1 MspI, GSTM1 and GSTT1 gene polymorphisms were investigated in 134 Ovambo and 207 Mongolians, and the results were compared with those from previous studies. Using polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) the frequency of CYP1A1 MspI mutation was determined. The multiplex PCR was used to determine the GSTM1 and GSTT1 polymorphism. The frequencies of wild-type, heterozygous variant and homozygous variant of the CYP1A1 MspI genotypes were 72.4%, 25.4% and 2.2%, and 22.7%, 55.6% and 21.7% in the Ovambos and Mongolians, respectively. The frequencies of GSTM1 (null) and GSTT1 (null) genotypes were 11.2% and 35.8%, and 46.4% and 25.6% in the Ovambos and Mongolians, respectively. The CYP1A1 MspI and GSTT1 (null) genotype distribution of the Ovambos was similar to that of African-Americans and some Caucasians. In contrast, the GSTM1 (null) genotype distribution was different from that of all other populations. Among Mongolians, the CYP1A1 MspI polymorphism showed the highest mutation frequencies, GSTM1 (null) was similar to that of East Asians, and GSTT1 (null) was different from that of almost all the Asians examined.