Pairings of lever and food induce Pavlovian conditioned approach of sign-tracking and goal-tracking in C57BL/6 mice.
Pairings of lever and food induce Pavlovian conditioned approach of sign-tracking and goal-tracking in C57BL/6 mice.
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杠杆和食物的配对在 C57BL/6 小鼠中诱导巴甫洛夫条件性信号跟踪和目标跟踪方法。
DOI：
10.1016/j.bbr.2011.10.021
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发表时间：
2012
影响因子：
2.7
通讯作者：
Yu,Lei
中科院分区：
文献类型：
--
作者：
Tomie,Arthur;Lincks,Michelle;Nadarajah,SteffiD;Pohorecky,LarissaA;Yu,Lei
在大鼠中，巴甫洛夫符号追踪已被广泛评估为药物成瘾和其他成瘾行为中的强迫性模型，但在小鼠中尚未探索，小鼠是一种具有丰富遗传修饰模型的物种，这使得研究基因-行为关系成为可能。在遗传学研究中最常用的小鼠品系C57 BL/6小鼠中，重复配对的杠杆条件刺激（CS）和食物非条件刺激（US）诱导了信号追踪条件反应（ST CR）的巴甫洛夫条件反射（Pavlovian conditioning），巴甫洛夫条件反射（英语：Pavlovian conditioning of goal tracking conditioned response，GT CR）针对食品US被递送的食品槽的位置的接近响应的性能。CS-US配对组在第15-16次治疗期间进行的ST CR和GT CR比伪条件反射对照组更多，伪条件反射对照组随机接受杠杆CS和食物US。在第15-16阶段，配对组中的所有小鼠进行的GT CR比ST CR多，回归分析显示个体受试者进行ST CR的倾向与GT CR之间存在正相关。在第15-16阶段进行更多ST CR的小鼠产生更高的血浆皮质酮水平。这些数据表明，稳定和可靠的收购和维护的ST CR性能和GT CR性能在小鼠中，但是，与大鼠不同，ST CR和GT CR没有相反的变化在受试者。皮质酮释放，一种易受药物滥用影响的病理生理学标志物，与ST CR表现呈正相关。
In rats, Pavlovian sign-tracking has been extensively evaluated as a model of compulsiveness in drug addiction and other addictive behaviors, but it remains unexplored in mice, a species with a wealth of genetically modified models, which makes it possible to examine gene-behavior relationships. In C57BL/6 mice, the most commonly used mouse strain for genetic studies, repeated pairings of lever conditioned stimulus (CS) with food unconditioned stimulus (US) induced Pavlovian conditioning of sign-tracking conditioned response (ST CR) performance of lever CS-directed approach, and Pavlovian conditioning of goal-tracking conditioned response (GT CR) performance of approach responses directed at the location of the food trough where the food US was delivered. The CS–US Paired group performed more ST CRs and more GT CRs during sessions 15–16 than did pseudoconditioning controls which received the lever CS and food US randomly with respect to one another. During sessions 15–16, all mice in the Paired group performed more GT CRs than ST CRs, and regression analysis revealed a positive relationship between an individual subject's tendency to perform ST CRs and GT CRs. The mice that performed more ST CRs during sessions 15–16 yielded higher plasma corticosterone levels. These data reveal stable and reliable acquisition and maintenance of ST CR performance and GT CR performance in mice; however, unlike in rats, ST CRs and GT CRs did not vary inversely within subjects. Corticosterone release, a pathophysiological marker of vulnerability to drug abuse, was positively related to ST CR performance.