Cow manure as additive to a DMBR for stable and high-rate digestion of food waste: Performance and microbial community
Cow manure as additive to a DMBR for stable and high-rate digestion of food waste: Performance and microbial community
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牛粪作为 DMBR 的添加剂，可稳定、高效地消化食物垃圾：性能和微生物群落
DOI：
10.1016/j.watres.2019.115099
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发表时间：
2020-01-01
期刊：
影响因子：
12.8
通讯作者：
Yuan, Honglin
中科院分区：
文献类型：
--
作者：
Xing, Bao-Shan;Han, Yule;Yuan, Honglin
在厌氧条件下，将牛粪（CM）添加到动态膜生物反应器（DMBR）中，以提高有机负荷（OLRs），使有机负荷从1.07 g COD/L/d增加到11.9 g COD/L/d，连续300 d以上。在FW/CM比为3.5：1（基于挥发性固体）时，混合液pH始终高于8.0，并且即使在11.9g COD/L/天的最高OLR（相应地，水力停留时间为10天，固体停留时间为15.5天）下也没有发生明显的挥发性脂肪酸（VFA）积累，表明非常稳定的操作条件，其导致平均CH 4产率高达250 mL/g COD和CH 4产率高达2.71L CH 4/L/天。难降解有机组分纤维素、半纤维素和木质素的有效降解率分别为78.3%、58.8%和47.5%。根据实验结果，计算了厌氧消化反应率，特别是水解率，这通常是限制因素。此外，高木质纤维素酶含量和辅酶F-420水平，沿着原料中纤维素结晶度从72.6%降低到16.4%，提供了通过CM添加增强的生物活性的强有力证据。通过高通量测序分析，从DMBR污泥中鉴定出更丰富多样的细菌、古菌和真菌属。随着CM的加入，木质纤维素的生物降解可能会产生足够的H-2和CO2的氢营养产甲烷菌，如甲烷球菌，甲烷微球菌，甲烷杆菌，这是高度耐受铵抑制，然后升高的铵水平将提供高缓冲能力的DMBR，从而确保高速率FW消化和CH 4生产的稳定条件。(C)2019爱思唯尔有限公司版权所有。
Cow manure (CM) was added to a dynamic membrane bioreactor (DMBR) operated under anaerobic condition for enhancing food waste (FW) digestion for over 300 days with stepwise increase of organic loading rates (OLRs) from 1.07 to 11.9 g COD/L/day. At a FW/CM ratio of 3.5:1 (based on volatile solids), the mixed liquor pH was always above 8.0 and no apparent volatile fatty acids (VFAs) accumulation occurred even at the highest OLR of 11.9 g COD/L/day (hydraulic retention time as 10 days and solid retention time as 15.5 days, correspondingly), indicating a very stable operation condition which resulted in an average CH4 yield as high as 250 mL/g COD and CH4 production as high as 2.71 L CH4/L/day. The hardly biodegradable organic components, such as cellulose, hemicellulose, and lignin, were effectively degraded by 78.3%, 58.8%, and 47.5%, respectively. Significantly high anaerobic digestion reaction ratios, especially the hydrolysis ratio which is usually the limiting factor, were calculated based on experimental results. Furthermore, the high lignocellulase contents and coenzyme F-420 levels, along with the decrease of cellulose crystallinity from 72.6% to 16.4% in the feedstock, provided strong evidence of an enhanced biological activity by CM addition. By high-throughput sequencing analysis, more abundant and diverse bacterial, archaeal, and fungal genera were identified from the DMBR sludge. With CM addition, the biodegradation of lignocellulose might have produced sufficient H-2 and CO2 for the hydrogenotrophic methanogens such as Methanoculleus, Methanomassiliicoccus, and Methanobacterium, which were highly tolerant to ammonium inhibition, and then the elevated ammonium level would have provided high buffering capacity in the DMBR thus ensuring a stable condition for high rate FW digestion and CH4 production. (C) 2019 Elsevier Ltd. All rights reserved.