Analysis of the green fraction of humic acids
Analysis of the green fraction of humic acids
复制标题
腐植酸绿色部分的分析
DOI：
10.1111/j.1365-2389.1996.tb01390.x
复制
发表时间：
1996
影响因子：
4.2
通讯作者：
S. Kuwatsuka
中科院分区：
文献类型：
--
作者：
A. Watanabe;H. Fujimori;Y. Nagai;T. Miyajima;S. Kuwatsuka
通过Sephadex G-50凝胶色谱分离腐殖酸的绿色组分Pg。将第一次层析得到的粗品进行重复层析，得到一个尖锐的峰（G1）和两个宽的带（G2），G1和G2中Pg的回收率分别为16.2%和14.5%。G1和G2在碱性溶液中的紫外-可见光谱在615、570、450和280 nm处都有明显的吸收峰。G1和G2的重均分子量估计值分别为9.7 x 10 3和1.23 x 10 4。在1H-NMR谱中，已知为Pg的发色团的4，9-二羟基-二萘嵌苯-3，10-醌（DHPQ）核的羟基的百分比分别为总H的4.1%和4.5%。根据这些值和H/C比值，DHPQ-C估计为总C的28%。使用反门控去耦获得的13 C-NMR谱产生40-41%的芳族C，这表明存在除DHPQ以外的芳环。IR、13 C-和1H-NMR谱表明，两种纯化的Pg样品在烷基和多糖组分的含量上存在很大差异。
The green fraction of humic acids (HAs), Pg, was fractionated by gel chromatography on Sephadex G-50. Repeated chromatography of the crude Pg obtained by the first chromatography of HA yielded a sharp peak (G 1 ) and two broad bands (G 2 ) of purified Pg. The recovery of Pg in G 1 and G 2 as percentages of the total Pg content of the HA were 16.2 and 14.5%, respectively. Distinct peaks were detected at 615, 570, 450 and 280 nm in the UV-visible spectra of both G 1 and G 2 in alkali solutions. Estimates of weight-average molecular weight were 9.7 x 10 3 for G 1 and 1.23 x 10 4 for G 2 . In the 1 H-NMR spectra the percentage of hydroxyl groups of 4,9-dihydroxy-perylene-3,10-quinone (DHPQ) nuclei, which are known to be the chromophore of Pg, was 4.1 and 4.5% of the total H, respectively. Based on these values and the H/C ratios, DHPQ-C was estimated as 28% of total C. 13 C-NMR spectra obtained using inverse-gated decoupling yielded 40-41% aromatic C, which suggested the presence of aromatic rings other than DHPQ. All of the IR, 13 C- and 1 H-NMR spectra indicated that the two purified Pg samples largely differed from each other in their contents of alkyl and polysaccharide components.