Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo
Antibacterial and anti-inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo
复制标题
医用导管上壳聚糖/铜复合涂层的抗菌和抗炎活性：体外和体内
DOI：
10.1002/jbm.b.35047
复制
发表时间：
2022-03-07
影响因子：
3.4
通讯作者：
Xu, Xiaohe
中科院分区：
文献类型：
--
作者：
Gu, Guisong;Erisen, Deniz Eren;Xu, Xiaohe
采用气动喷涂的方法在硅橡胶表面制备了铜离子接枝壳聚糖涂层。对涂层的表面性能、表面形貌、组成、铜的释放行为、抗菌和抗炎活性进行了研究和讨论。通过扫描电子显微镜(SEM)和接触角测量对其表面性质、组成和形貌进行了研究。用大肠杆菌和金黄色葡萄球菌混悬液进行了体外抑菌试验。此外，用场发射扫描电子显微镜观察了生物膜的形貌。为了评价涂层的体内抗炎活性和生物安全性，将优化后的涂层样品和对照组植入小鼠背部皮下。通过注射细菌悬浮液建立细菌环境模型。植入5d后，观察导管表面及周围组织的形态及细菌黏附情况。体外实验结果表明，随着壳聚糖铜含量的增加，硅橡胶表面附着的细菌数量减少，抗炎效果增强。至于体内结果，植入5d后，所有未注射金黄色葡萄球菌组的导管周围组织均未见明显炎症反应。注射壳聚糖/铜包被组的炎症反应、粘附菌数量明显少于未注射铜的其他注射组，无明显炎症反应。结果表明，铜改性壳聚糖涂层在医用导管上具有良好的抗菌抗炎活性。
Copper ions (Cu) grafted chitosan coating was prepared using the pneumatic spraying method on the silicone rubber surface. Coating's surface properties, morphology, composition, Cu releasing behavior, antibacterial, and anti-inflammatory activities are investigated and discussed. Surface properties, composition, and morphology were investigated by scanning electron microscopy (SEM) and contact angle measurements. The antibacterial activity has been tested with Escherichia coli and Staphylococcus aureus suspensions in vitro. Besides, the morphology of the biofilm was inspected with a field emission SEM. To evaluate the anti-inflammatory activity and biosafety of the coating in vivo, the optimized coating samples and control groups were implanted subcutaneously into the back of mice. The bacterial environment model was established by injection of the bacterial suspension. The morphology and bacterial adhered on the surface of catheters and the surrounding tissues were analyzed after 5 days of implantation. As in vitro results, the number of adhered bacterial on the surface of the silicon rubber surface was decreased, and the anti-inflammatory rate was increased by the intensify of the Cu content in chitosan coating. As for in vivo results, after 5 days of implantation, there was no evident inflammation in the surrounding tissues of all catheters in all without the S. aureus injected group. In the injected chitosan/Cu coated group; the inflammation, the number of the adhered bacteria were observed less than other injected samples without Cu; no inflammation were noticeable. Results indicate that the Cu-modified chitosan coating can confer excellent antibacterial and anti-inflammatory activity as applied on medical catheters.