Quality control of photosystem II: Cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids
Quality control of photosystem II: Cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids
复制标题
DOI：
10.1016/j.bbabio.2007.05.001
复制
发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Yamamoto, Yasusi
中科院分区：
文献类型：
--
作者：
Komayama, Keisuke;Khatoon, Mahbuba;Yamamoto, Yasusi
菠菜类囊体受到中度热胁迫(40℃，30min)后，D1蛋白发生裂解，产生N端23 kDa的片段，C端9 kDa的片段，以及D1蛋白的聚集。拟南芥类囊体基质中富含拟南芥FtsH2蛋白同源酶，该酶负责降解受损的D1蛋白。类囊体对热胁迫的响应经历了两个过程：基质类囊体中D1蛋白的去磷酸化和基粒中磷酸化的D1蛋白的聚集。热胁迫还诱导外源PsbO、P和Q蛋白从光系统II中释放，这对D1的降解和聚集有显著影响。D1蛋白的切割和聚集似乎是两个交替的过程，受蛋白质磷酸化/去磷酸化、FtsH的分布和类囊体体完整性的影响。(C)2007爱思唯尔B.V保留所有权利。
Moderate heat stress (40 degrees C, 30 min) on spinach thylakoids induced cleavage of the D1 protein, producing an N-terminal 23-kDa fragment, a C-terminal 9-kDa fragment, and aggregation of the D1 protein. A homologue of Arabidopsis FtsH2 protease, which is responsible for degradation of the damaged D1 protein, was abundant in the stroma thylakoids. Two processes occurred in the thylakoids in response to heat stress: dephosphorylation of the D1 protein in the stroma thylakoids, and aggregation of the phosphorylated D1 protein in the grana. Heat stress also induced the release of the extrinsic PsbO, P and Q proteins from Photosystem II, which affected D1 degradation and aggregation significantly. The cleavage and aggregation of the D1 protein appear to be two alternative processes influenced by protein phosphorylation/dephosphorylation, distribution of FtsH, and intactness of the thylakoids. (c) 2007 Elsevier B.V All rights reserved.