Experimental and Pathalogical study of Pistacia atlantica, butyrate, Lactobacillus casei and their combination on rat ulcerative colitis model
Experimental and Pathalogical study of Pistacia atlantica, butyrate, Lactobacillus casei and their combination on rat ulcerative colitis model
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DOI：
10.1016/j.prp.2016.02.024
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Abdollahi, Mohammad
中科院分区：
文献类型：
--
作者：
Gholami, Mahdi;Ghasemi-Niri, Seyedeh Farnaz;Abdollahi, Mohammad
本研究评价了阿月浑子、丁酸盐、干酪乳杆菌、乳酸菌、casei），尤其是它们对2，4，6-三硝基苯磺酸（TNBS）诱导的大鼠结肠炎模型的联合治疗。将大鼠分为7组。四组分别口服大西洋青霉、丁酸盐、L. casei和三种试剂的组合连续10天。其余组为阴性和阳性对照以及假手术组。进行了宏观和组织病理学检查沿着结肠氧化应激的特异性生物标志物髓过氧化物酶（MPO）的测定。与对照组相比，联合治疗在病理评分和MPO活性降低方面显示出结肠炎的显著缓解（55%，p = 0.0009）。同时，肉眼观察，如粪便粘稠度、组织和组织病理学评分（水肿、坏死和中性粒细胞浸润）得到改善。虽然单用大西洋原杆菌、丁酸杆菌和L.酪蛋白在降低结肠氧化应激标志物方面部分有益，联合治疗更有效。总之，联合治疗能够降低结肠炎的严重程度，这从生化标志物中可以清楚地看出。未来的研究必须关注这种组合在人类溃疡性结肠炎管理中的临床效果。进一步的分子和信号通路研究将有助于理解结肠炎和炎症性疾病治疗中涉及的机制。(C)2016年爱思唯尔有限公司。All rights reserved.
This study evaluated the effects of Pistacia atlantica (P. atlantica), butyrate, Lactobacillus casei (L. casei) and especially their combination therapy on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced rat colitis model. Rats were divided into seven groups. Four groups received oral P. atlantica, butyrate, L. casei and the combination of three agents for 10 consecutive days. The remaining groups were negative and positive controls and a sham group. Macroscopic and histopathological examinations were carried out along with determination of the specific biomarker of colonic oxidative stress, the myeloperoxidase (MPO). Compared with controls, the combination therapy exhibited a significant alleviation of colitis in terms of pathological scores and reduction of MPO activity (55%, p = 0.0009). Meanwhile, the macroscopic appearance such as stool consistency, tissue and histopathological scores (edema, necrosis and neutrophil infiltration) were improved. Although single therapy by each P. atlantica, butyrate, and L. casei was partially beneficial in reduction of colon oxidative stress markers, the combination therapy was much more effective. In conclusion, the combination therapy was able to reduce the severity of colitis that is clear from biochemical markers. Future studies have to focus on clinical effects of this combination in management of human ulcerative colitis. Further molecular and signaling pathway studies will help to understand the mechanisms involved in the treatment of colitis and inflammatory diseases. (C) 2016 Elsevier GmbH. All rights reserved.