Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells
Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells
复制标题
DOI：
10.1242/dev.015412
复制
发表时间：
2008-05-01
期刊：
影响因子：
4.6
通讯作者：
Ruhrberg, Christiana
中科院分区：
文献类型：
--
作者：
Schwarz, Quenten;Vieira, Joaquim M.;Ruhrberg, Christiana
神经毡蛋白（NRP）受体及其3类信号蛋白（SEMA 3）配体在轴突引导中发挥着公认的作用，NRP 1、NRP 2、SEMA 3A或SEMA 3F的缺失会导致去纤维束和外周投射神经生长锥引导错误。在这里，我们报告说，NRP 1或NRP 2的损失也损害感觉神经元定位在小鼠头部，这种缺陷是不适当的颅神经嵴细胞迁移的结果。具体来说，神经嵴细胞移动到正常的无嵴的领土之间的三叉神经和舌骨神经嵴流和招聘的感觉神经元从耳基板，这些异位神经元，然后延伸轴突之间的三叉神经和面声神经节。此外，我们发现NRP 1和NRP 2合作，引导颅神经嵴细胞和位置的感觉神经元;因此，在SEMA 3/NRP信号的情况下，颅神经系统的分割丢失。我们的结论是神经纤毛蛋白在感觉神经系统中发挥多种作用，指导颅神经嵴细胞，定位感觉神经元和组织他们的轴突投射。
Neuropilin (NRP) receptors and their class 3 semaphorin (SEMA3) ligands play well-established roles in axon guidance, with loss of NRP1, NRP2, SEMA3A or SEMA3F causing defasciculation and errors in growth cone guidance of peripherally projecting nerves. Here we report that loss of NRP1 or NRP2 also impairs sensory neuron positioning in the mouse head, and that this defect is a consequence of inappropriate cranial neural crest cell migration. Specifically, neural crest cells move into the normally crest-free territory between the trigeminal and hyoid neural crest streams and recruit sensory neurons from the otic placode; these ectopic neurons then extend axons between the trigeminal and facioacoustic ganglia. Moreover, we found that NRP1 and NRP2 cooperate to guide cranial neural crest cells and position sensory neurons; thus, in the absence of SEMA3/NRP signalling, the segmentation of the cranial nervous system is lost. We conclude that neuropilins play multiple roles in the sensory nervous system by directing cranial neural crest cells, positioning sensory neurons and organising their axonal projections.