Influence of lipids on the interfacial disposition of respiratory syncytical virus matrix protein.
Influence of lipids on the interfacial disposition of respiratory syncytical virus matrix protein.
复制标题
脂质对呼吸道合胞病毒基质蛋白界面分布的影响。
DOI：
10.1021/la104041n
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
McPhee HK
中科院分区：
文献类型：
--
作者：
McPhee HK
已使用无标记方法测定了来自单负病毒家族包膜病毒的基质蛋白与代表病毒包膜的脂质结合的倾向，包括在空气-水界面处的脂质膜上进行的张力测定和布鲁斯特角显微镜检查，以及在转移到经OTS处理的硅片上的单层上进行的原子力显微镜检查。这使得影响蛋白质相对于脂质界面的配置的因素得以表征。在没有鞘磷脂的情况下，呼吸道合胞病毒基质蛋白在空气-水界面穿透由磷酸胆碱与磷酸乙醇胺或胆固醇的混合物组成的单层。在由鞘磷脂、1，2-二油酰基-sn-甘油-3-磷酸胆碱和胆固醇组成的三元混合物中，该蛋白表现出两种独立的行为：（1）与鞘磷脂富集结构域表面的外周缔合和（2）渗透鞘磷脂贫乏结构域。蛋白质与磷酸胆碱和磷酸乙醇胺的混合物的长时间孵育导致形成均匀直径的螺旋蛋白质组装体，其证明蛋白质组装成丝状形式的固有倾向。
The propensity of a matrix protein from an enveloped virus of theMononegaviralesfamily to associate with lipids representative of the viral envelope has been determined using label-free methods, including tensiometry and Brewster angle microscopy on lipid films at the air−water interface and atomic force microscopy on monolayers transferred to OTS-treated silicon wafers. This has enabled factors that influence the disposition of the protein with respect to the lipid interface to be characterized. In the absence of sphingomyelin, respiratory syncytial virus matrix protein penetrates monolayers composed of mixtures of phosphocholines with phosphoethanolamines or cholesterol at the air−water interface. In ternary mixtures composed of sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol, the protein exhibits two separate behaviors: (1) peripheral association with the surface of sphingomyelin-rich domains and (2) penetration of sphingomyelin-poor domains. Prolonged incubation of the protein with mixtures of phosphocholines and phosphoethanolamines leads to the formation of helical protein assemblies of uniform diameter that demonstrate an inherent propensity of the protein to assemble into a filamentous form.