Molecular cloning of actin filament-associated protein: a putative adaptor in stretch-induced Src activation
Molecular cloning of actin filament-associated protein: a putative adaptor in stretch-induced Src activation
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DOI：
10.1152/ajplung.00492.2001
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发表时间：
2002-08-01
影响因子：
4.9
通讯作者：
Liu, MY
中科院分区：
文献类型：
--
作者：
Lodyga, M;Bai, XH;Liu, MY
机械拉伸诱导的c-Src活化是拉伸诱导胎鼠肺细胞增殖信号转导的重要步骤。这一过程似乎是通过肌动蛋白丝相关蛋白（AFAP）介导的，该蛋白由最初克隆自鸡的基因编码。在本研究中，我们从胎鼠肺中克隆了大鼠AFAP基因。其mRNA和蛋白在不同组织中有差异表达。该蛋白在胎鼠肺上皮细胞和成纤维细胞中与肌动蛋白丝共定位。机械拉伸在最初几分钟内增加了大鼠AFAP的酪氨酸磷酸化及其与c-Src的结合。Src SH2和SH3结合基序在AFAP蛋白中高度保守（从鸡、大鼠到人）。根据AFAP蛋白的分子结构，我们推测它是机械拉伸诱导c-Src活化的接头。提出了一种新的机械接收模型。
Mechanical stretch-induced activation of c-Src is an important step for signal transduction of stretch-induced fetal rat lung cell proliferation. This process appears to be mediated through actin filament-associated protein (AFAP), encoded by a gene originally cloned from the chicken. In the present study, we cloned the rat AFAP gene from fetal rat lungs. Its mRNA and protein are differentially expressed among various tissues. The protein is colocalized with actin filaments in fetal rat lung epithelial cells and fibroblasts. Mechanical stretch increased tyrosine phosphorylation of rat AFAP and its binding to c-Src within the initial several minutes. Src SH2 and SH3 binding motifs are highly conserved in the AFAP proteins (from chicken, rat to human). On the basis of the molecular structure of AFAP protein, we speculate that it is an adaptor in mechanical stretch-induced activation of c-Src. A novel model of mechanoreception is proposed.