Bovine Astrovirus Surveillance in Uruguay Reveals High Detection Rate of a Novel Mamastrovirus Species
Bovine Astrovirus Surveillance in Uruguay Reveals High Detection Rate of a Novel Mamastrovirus Species
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DOI：
10.3390/v12010032
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发表时间：
2020-01-01
期刊：
影响因子：
4.7
通讯作者：
Colina, Rodney
中科院分区：
文献类型：
--
作者：
Castells, Matias;Bertoni, Estefany;Colina, Rodney
牛肠道星状病毒（BoAstV）在美国的流行、致病性和遗传多样性等方面的研究较少。这项工作的目的是描述乌拉圭奶牛肠道BoAstV的患病率和遗传多样性。在2015年7月至2017年5月期间，共收集了457头奶牛的粪便和43头奶牛的肠道内容物，并通过RT-PCR进行了检测，随后对聚合酶和衣壳区域进行了测序和系统发育分析。26%（128/500）的样本呈阳性。鉴定了Mamastrovirus属内的三种不同种属，包括Mamastrovirus 28、Mamastrovirus 33（各3份样品）和未分类的Mamastrovirus种属（19份样品）。未分类的种被表征为新的Mamastrovirus种。BoAstV在乌拉圭奶牛中传播，具有高度遗传多样性。牛肠道BoAstV感染的最终临床病理学意义需要进一步研究。
Viral infections affecting cattle lead to economic losses to the livestock industry worldwide, but little is known about the circulation, pathogenicity and genetic diversity of enteric bovine astrovirus (BoAstV) in America. The aim of this work was to describe the prevalence and genetic diversity of enteric BoAstV in dairy cattle in Uruguay. A total of 457 fecal and 43 intestinal contents from dairy calves were collected between July 2015 and May 2017 and tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase and capsid regions. Twenty-six percent (128/500) of the samples were positive. Three different species within the Mamastrovirus genus were identified, including Mamastrovirus 28, Mamastrovirus 33 (3 samples each) and an unclassified Mamastrovirus species (19 samples). The unclassified species was characterized as a novel Mamastrovirus species. BoAstV circulates in Uruguayan dairy cattle with a high genetic diversity. The eventual clinicopathological significance of enteric BoAstV infection in cattle needs further investigation.