Enhanced Rat Islet Function and Survival In Vitro Using a Biomimetic Self-Assembled Nanomatrix Gel
Enhanced Rat Islet Function and Survival In Vitro Using a Biomimetic Self-Assembled Nanomatrix Gel
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DOI：
10.1089/ten.tea.2010.0151
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发表时间：
2011-02-01
影响因子：
4.1
通讯作者：
Jun, Ho-Wook
中科院分区：
文献类型：
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作者：
Lim, Dong-Jin;Antipenko, Sergey V.;Jun, Ho-Wook
肽两亲物（PA）是一种基于肽的生物材料，它能够自组装成纳米结构的凝胶状支架，模拟天然细胞外基质的化学和生物学复杂性。为了评估PA支架在体外改善胰岛功能和存活的能力，将大鼠胰岛分为三组进行培养：（1）空白组：将分离的大鼠胰岛培养在未经组织培养处理的12孔板中；（2）插入组：将分离的大鼠胰岛培养在改良的插入式小室中；（3）纳米基质组：将分离的大鼠胰岛包裹在PA纳米基质凝胶内，并培养在改良的插入式小室中。在14天的时间里，空白组和插入组的胰岛素分泌均显著减少，而纳米基质组则维持了葡萄糖刺激的胰岛素分泌。此外，在纳米基质凝胶中的整个胰岛在长达14天的时间里双硫腙染色呈阳性，这表明其更好地维持了葡萄糖刺激的胰岛素产生。荧光素二乙酸酯/碘化丙啶染色结果也证实，空白组和插入组在7天后出现坏死，而PA纳米基质凝胶在14天后仍维持胰岛的活力。因此，这些结果证明了PA作为一种中间支架在提高胰岛移植效果方面的潜力。
Peptide amphiphile (PA) is a peptide-based biomaterial that can self-assemble into a nanostructured gel-like scaffold, mimicking the chemical and biological complexity of natural extracellular matrix. To evaluate the capacity of the PA scaffold to improve islet function and survival in vitro, rat islets were cultured in three different groups-(1) bare group: isolated rat islets cultured in a 12-well nontissue culture-treated plate; (2) insert group: isolated rat islets cultured in modified insert chambers; (3) nanomatrix group: isolated rat islets encapsulated within the PA nanomatrix gel and cultured in modified insert chambers. Over 14 days, both the bare and insert groups showed a marked decrease in insulin secretion, whereas the nanomatrix group maintained glucose-stimulated insulin secretion. Moreover, entire islets in the nanomatrix gel stained positive for dithizone up to 14 days, indicating better maintained glucose-stimulated insulin production. Fluorescein diacetate/propidium iodide staining results also verified necrosis in the bare and insert groups after 7 days, whereas the PA nanomatrix gel maintained islet viability after 14 days. Thus, these results demonstrate the potential of PAs as an intermediary scaffold for increasing the efficacy of pancreatic islet transplantation.