A MACROMOLECULAR INHIBITOR OF THE ANTIZYME TO ORNITHINE DECARBOXYLASE
A MACROMOLECULAR INHIBITOR OF THE ANTIZYME TO ORNITHINE DECARBOXYLASE
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DOI：
10.1042/bj2040647
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发表时间：
1982-01-01
影响因子：
4.1
通讯作者：
HAYASHI, S
中科院分区：
文献类型：
--
作者：
FUJITA, K;MURAKAMI, Y;HAYASHI, S
在大鼠肝提取物中发现了一种抑制抗鸟氨酸脱羧酶（ODC）活性的大分子因子。抗酶抑制因子是热不稳定的，非扩散性的，并且与ODC具有相似的分子大小。抗酶抑制剂重新激活ODC，已被灭活的抗酶，显然是通过取代ODC与抗酶的复合物。因此，抗酶抑制剂可用于测定体外形成的无活性ODC-抗酶复合物的量。当用这种方法测定时，复合物显示出在ODC之前从Sephadex G-100柱洗脱。ODC活性显着增加时，观察到的抗酶抑制剂被添加到粗肝提取物，从大鼠已被注射1，3-二氨基丙烷引起衰减的ODC活性，这表明存在的非活性ODC-抗酶复合物的提取物。
A macromolecular factor that inhibits the activity of the antizyme to ornithine decarboxylase (ODC) was found in rat liver extracts. The factor, antizyme inhibitor, was heat-labile, non-diffusable and of similar molecular size to ODC. The antizyme inhibitor reactivated ODC that had been inactivated by antizyme, apparently by replacing ODC in a complex with antizyme. Therefore the antizyme inhibitor can be used to assay the amount of inactive ODC-antizyme complex formed in vitro. When assayed by this method, the complex was shown to be eluted before ODC from a Sephadex G-100 column. Significant increase in ODC activity was observed when the antizyme inhibitor was added to crude liver extracts from rats that had been injected with 1,3-diaminopropane to cause decay of ODC activity, suggesting the presence of inactive ODC-antizyme complex in the extracts.