3 TYPES OF AMYLOID PROTEIN-PRECURSOR MESSENGER-RNA IN HUMAN-BRAIN - THEIR DIFFERENTIAL EXPRESSION IN ALZHEIMERS-DISEASE
3 TYPES OF AMYLOID PROTEIN-PRECURSOR MESSENGER-RNA IN HUMAN-BRAIN - THEIR DIFFERENTIAL EXPRESSION IN ALZHEIMERS-DISEASE
复制标题
DOI：
10.1016/s0006-291x(88)80273-0
复制
发表时间：
1988-12-15
影响因子：
3.1
通讯作者：
ITO, H
中科院分区：
文献类型：
--
作者：
TANAKA, S;NAKAMURA, S;ITO, H
应用Northern印迹法在正常人和阿尔茨海默病患者大脑中检测到三种由选择性剪接产生的淀粉样蛋白前体（APP） mRNA。这些mrna编码的APP695由695个氨基酸组成，APP751在APP695中含有56个氨基酸插入物，与kunitz型胰蛋白酶抑制剂同源，APP770含有另外19个氨基酸插入物。另一种可能编码“APP714”的APP mRNA含有19个氨基酸插入物，在测试的大脑样本中未发现。定量分析发现，虽然3种mRNA的相对表达量在个体间存在差异，但与对照组相比，阿尔茨海默病中APP695和APP751 mRNA的表达量无显著变化，而APP770 mRNA的表达量在阿尔茨海默病中显著升高。
Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing were detected by Northern blotting in human brains, both control and Alzheimer''s disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer''s disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer''s disease.