Roles of very-long-chain fatty acids in compound leaf patterning in Medicago truncatula
Roles of very-long-chain fatty acids in compound leaf patterning in Medicago truncatula
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极长链脂肪酸在蒺藜苜蓿复合叶型中的作用
DOI：
10.1093/plphys/kiad006
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发表时间：
2023
期刊：
影响因子：
7.4
通讯作者：
Chuanen Zhou
中科院分区：
文献类型：
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作者：
Hongfeng Wang;Zhichao Lu;Yiteng Xu;Jing Zhang;Lu Han;Maofeng Chai;Zeng-Yu Wang;Xianpeng Yang;Shiyou Lu;Jianhua Tong;Langtao Xiao;Jiangqi Wen;Kirankumar S Mysore;Chuanen Zhou
抽象的。植物角质层是由疏水性角质层蜡和角质组成。极长链脂肪酸（VLCFA）是表皮蜡和质膜的组分，并参与器官形态发生。通过筛选烟草细胞转座因子1（Tnt 1）标记的桶形苜蓿（Medicago truncatula）突变体群体，获得了2种具有不开放花表型的突变体，命名为unopened flower 1（uof 1）和uof 2。UOF 1和UOF 2都编码参与VLCFA和表皮蜡生物合成的酶。对突变体的比较分析表明，UOF 1的突变而不是UOF 2的突变导致M中小叶数量的增加。蒺藜UOF 1在茎尖分生组织和叶原基的最外层细胞中特异表达。uof 1突变体在VLCFAs介导的质膜完整性方面表现出缺陷，导致PIN-FORMED 1（PIN 1）直系同源物SMOOTH LEAF MARGIN 1（SLM 1）在M中的无序定位。蒺藜我们的工作表明，UOF 1介导的VLCFAs在L1中的生物合成是复叶模式的关键，这与M中生长素流出载体的极化有关。蒺藜
Abstract. Plant cuticles are composed of hydrophobic cuticular waxes and cutin. Very long-chain fatty acids (VLCFAs) are components of epidermal waxes and the plasma membrane and are involved in organ morphogenesis. By screening a barrelclover (Medicago truncatula) mutant population tagged by the transposable element of tobacco (Nicotiana tabacum) cell type1 (Tnt1), we identified two types of mutants with unopened flower phenotypes, named unopened flower1 (uof1) and uof2. Both UOF1 and UOF2 encode enzymes that are involved in the biosynthesis of VLCFAs and cuticular wax. Comparative analysis of the mutants indicated that the mutation in UOF1, but not UOF2, leads to increased number of leaflets in M. truncatula. UOF1 was specifically expressed in the outermost cell layer (L1) of the shoot apical meristem (SAM) and leaf primordia. The uof1 mutants displayed defects in VLCFAs-mediated plasma membrane integrity, resulting in the disordered localization of the PIN-FORMED1 (PIN1) ortholog SMOOTH LEAF MARGIN1 (SLM1) in M. truncatula. Our work demonstrates that the UOF1-mediated biosynthesis of VLCFAs in L1 is critical for compound leaf patterning, which is associated with the polarization of the auxin efflux carrier in M. truncatula.