Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures.
Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures.
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DOI：
10.1007/978-1-0716-1979-7_31
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发表时间：
2022-01-01
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影响因子：
--
通讯作者：
Doles, Jason D
中科院分区：
文献类型：
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作者：
Arneson-Wissink, Paige C;Doles, Jason D
离体培养原代肌肉干细胞是在受控环境中研究该细胞群的有用方法。原代肌肉干细胞对外界刺激的反应与永生化成肌细胞（C2C12细胞）不同，这使得肌肉干细胞的离体培养成为了解细胞对刺激反应的重要工具。离体培养的原代肌肉干细胞保留了它们在体内所具有的大部分特征，例如分化成多核结构和自我更新干细胞样群体的能力。在这一章中，我们描述了分离原代肌肉干细胞，控制分化成肌管，并使用IncuCyte活细胞成像和分析软件定量分化的方法。
Culturing primary muscle stem cells ex vivo is a useful method for studying this cell population in controlled environments. Primary muscle stem cells respond to external stimuli differently than immortalized myoblasts (C2C12 cells), making ex vivo culture of muscle stem cells an important tool in understanding cell responses to stimuli. Primary muscle stem cells cultured ex vivo retain a majority of the characteristics they possess in vivo such as the abilities to differentiate into multinucleated structures, and self-renew a stem cell-like population. In this chapter, we describe methods for isolating primary muscle stem cells, controlled differentiation into myotubes, and quantification of differentiation using IncuCyte live cell imaging and analysis software.