Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length
Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length
复制标题
人类 MCRS2 是一种细胞周期依赖性蛋白，与 LPTS/PinX1 结合并缩短端粒长度
DOI：
10.1016/j.bbrc.2004.02.166
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发表时间：
2004-04-16
影响因子：
3.1
通讯作者：
Zhao, MJ
中科院分区：
文献类型：
--
作者：
Song, H;Li, YL;Zhao, MJ
人LPTS/PinX 1是一种端粒酶抑制蛋白，它与端粒蛋白Pin 2/TRF 1和端粒酶催化亚基hTERT结合。为了探索可能参与端粒酶途径的蛋白质，我们以LPTS/PinX 1为诱饵进行酵母双杂交筛选。一个新的基因，MCRS 2，编码的同种型MCRS 1/p78和MSP 58的分离。MCRS 2蛋白的表达是细胞周期依赖性的，在非常早期的S期积累。MCRS 2与LPTS/PinX 1在体外、体内相互作用，并与LPTS/PinX 1在细胞中共定位。MCRS 2及其氨基端在体外抑制端粒酶活性，长期过表达MCRS 2可导致SMMC-7721细胞端粒逐渐缩短。我们的研究结果表明，MCRS 2可能是端粒维持和细胞周期调控之间的连接器。(C)2004年爱思唯尔公司All rights reserved.
Human LPTS/PinX1 is a telomerase-inhibitory protein, which binds to the telomere protein Pin2/TRF1 and the catalytic subunit hTERT of telomerase. To explore the proteins that might be involved in the telomerase pathway, we performed a yeast two-hybrid screening with LPTS/PinX1 as the bait. A novel gene, MCRS2, encoding for an isoform of MCRS1/p78 and MSP58 was isolated. The expression of MCRS2 protein is cell-cycle dependent, accumulating in the very early S phase. MCRS2 interacts with LPTS/PinX1 in vitro, in vivo and colocalizes with LPTS/PinX1 in cells. MCRS2 and its amino terminus inhibit telomerase activity in vitro and long-term overexpression of MCRS2 in SMMC-7721 cells results in a gradual and progressive shortening of telomeres. Our findings suggest that MCRS2 might be a linker between telomere maintenance and cell-cycle regulation. (C) 2004 Elsevier Inc. All rights reserved.