Two-photon fluorescence correlation spectroscopy of lipid-encapsulated fluorescent nanodiamonds in living cells
Two-photon fluorescence correlation spectroscopy of lipid-encapsulated fluorescent nanodiamonds in living cells
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DOI：
10.1364/oe.18.005896
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发表时间：
2010-03-15
期刊：
影响因子：
3.8
通讯作者：
Chang, Fu-Hsiung
中科院分区：
文献类型：
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作者：
Hui, Yuen Yung;Zhang, Bailin;Chang, Fu-Hsiung
用双光子荧光相关光谱法研究了荧光金刚石纳米颗粒在HeLa细胞中的动力学行为。荧光纳米金刚石（FND）是生物成像应用的一种优良荧光探针，但它们在细胞摄入后经常被捕获在核内体中。诱捕禁止在60秒的时间范围内进行FCS。在此，我们表明，脂质层内的FND的封装增强了细胞质中的颗粒的扩散超过一个数量级，和小至40 nm的颗粒可以单独探测与高图像对比度的双光子激发发光。该技术的发展与通过单光子激发的单粒子跟踪一起允许探测活细胞中单个FND的短期和长期动力学。(C)2010年美国光学学会
Dynamics of fluorescent diamond nanoparticles in HeLa cells has been studied with two-photon fluorescence correlation spectroscopy (FCS). Fluorescent nanodiamond (FND) is an excellent fluorescent probe for bioimaging application, but they are often trapped in endosomes after cellular uptake. The entrapment prohibits FCS from being performed in a time frame of 60 s. Herein, we show that the encapsulation of FNDs within a lipid layer enhances the diffusion of the particles in the cytoplasm by more than one order of magnitude, and particles as small as 40 nm can be probed individually with high image contrast by two-photon excited luminescence. The development of the technique together with single particle tracking through one-photon excitation allows probing of both short-term and long-term dynamics of single FNDs in living cells. (C) 2010 Optical Society of America