Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane.
Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane.
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DOI：
10.1083/jcb.200508014
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发表时间：
2006-03-13
期刊：
影响因子：
--
通讯作者：
Gould SJ
中科院分区：
文献类型：
--
作者：
Booth AM;Fang Y;Fallon JK;Yang JM;Hildreth JE;Gould SJ
外泌体是分泌的、直径约100 nm的单膜细胞器。它们的生物发生通常被认为发生在两步过程中，涉及（1）在内体的限制膜处向外囊泡出芽（向外=远离细胞质），其产生腔内囊泡，随后（2）内体-质膜融合，其将这些内部囊泡作为外来体释放到细胞外环境中。在这项研究中，我们提出的证据表明，某些细胞，包括Jurkat T细胞，具有离散的结构域的质膜是丰富的外泌体和内体蛋白，保留内体的属性向外囊泡出芽，并作为网站的直接外泌体生物发生。据推测，逆转录病毒利用外来体生物发生途径形成感染性颗粒。为了支持这一点，我们发现Jurkat T细胞将HIV的关键出芽因子HIV Gag引导到质膜的这些内体样结构域，并将HIV Gag从细胞分泌到外泌体中。
Exosomes are secreted, single membrane organelles of ∼100 nm diameter. Their biogenesis is typically thought to occur in a two-step process involving (1) outward vesicle budding at limiting membranes of endosomes (outward = away from the cytoplasm), which generates intralumenal vesicles, followed by (2) endosome–plasma membrane fusion, which releases these internal vesicles into the extracellular milieu as exosomes. In this study, we present evidence that certain cells, including Jurkat T cells, possess discrete domains of plasma membrane that are enriched for exosomal and endosomal proteins, retain the endosomal property of outward vesicle budding, and serve as sites of immediate exosome biogenesis. It has been hypothesized that retroviruses utilize the exosome biogenesis pathway for the formation of infectious particles. In support of this, we find that Jurkat T cells direct the key budding factor of HIV, HIV Gag, to these endosome-like domains of plasma membrane and secrete HIV Gag from the cell in exosomes.
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