Association of TERT gene polymorphisms with clinical benign prostatic hyperplasia in a Chinese Han population of the Northwest region.
Association of TERT gene polymorphisms with clinical benign prostatic hyperplasia in a Chinese Han population of the Northwest region.
复制标题
西北地区汉族人群TERT基因多态性与临床良性前列腺增生的关系
DOI：
10.21037/tau-20-1032
复制
发表时间：
2021-03
影响因子：
2
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Fan G;Li K;Pang Y;Zhao Y;Tao Y;Gui H;Wang H;Svatek R;Rodriguez R;Wang Z
目的探讨中国西北地区汉族人群端粒酶基因（telomerase gene，TERT）rs 10078761、rs 12696304、rs 2853669、rs 16847897、rs 2736100、rs 10069690单核苷酸多态性与良性前列腺增生（benign prostate hyperplasia，BPH）的关系。方法选择150例BPH患者和150例健康老年男性作为研究对象。通过查找表法估计了该不匹配病例对照研究的样本量。同时收集患者的一般资料和疾病资料。仅收集健康对照受试者的年龄进行统计学校正。使用多重PCR +连接酶检测反应（LDR）检测基因型。分型结果与临床资料采用多元线性回归和Logistic回归进行统计学分析。Pearson相关性用于Hardy-Weinberg平衡。结果纳入人群符合Hardy-Weinberg平衡。相关性分析显示，SNP与BPH的发病风险无显著相关性。单倍型分析结果显示，4种单倍型（TCTGGT、TCTGTC、TGCCTC和TGTGTC）是BPH的危险因素。SNP rs 2853669是平滑肌型增生的独立危险因素。rs 2736100、rs 10078761和rs 10069690与BPH的严重程度相关，且处于连锁不平衡状态。结论TERT基因多态性决定了中国西北地区汉族人群BPH不同的疾病发展和病理表现。
Background To investigate the association between single nucleotide polymorphisms (rs10078761, rs12696304, rs2853669, rs16847897, rs2736100, rs10069690) of telomerase gene (TERT) and the risk clinical benign prostatic hyperplasia (BPH) in a Chinese Han population of the Northwest region. Methods A total of 150 BPH patients and 150 healthy older males from the northwest Chinese Han population were included in this study. The sample size for this unmatched case-control study was estimated by the look-up table method. Meanwhile, the general information and disease data of patients were collected. Age was only collected in healthy control subjects for statistical correction. Genotypes were detected using a multiplex PCR + ligase detection reaction (LDR). Typing results and clinical data were statistically analyzed using multiple linear regression and logistic regression. Pearson correlation was used for Hardy-Weinberg equilibrium. Results The included population is in Hardy-Weinberg equilibrium. There was no significant association between SNP and the risk of BPH by correlation analysis. However, 4 haplotypes (TCTGGT, TCTGTC, TGCCTC, and TGTGTC) were identified as risk factors of BPH by haplotype analysis. The SNP rs2853669 is an independent risk factor for smooth muscle type of hyperplasia. Besides, rs2736100, rs10078761, and rs10069690 which are in linkage disequilibrium are associated with the severity of BPH. Conclusions Polymorphism of the TERT gene determines the different disease development and pathological manifestations of BPH in the Chinese Han population the Northwest region.