Arbuscular mycorrhiza confers lead tolerance and uptake in Miscanthus sacchariflorus
Arbuscular mycorrhiza confers lead tolerance and uptake in Miscanthus sacchariflorus
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DOI：
10.1080/02757540.2018.1437150
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发表时间：
2018-02
影响因子：
2.5
通讯作者：
Animesh Sarkar;T. Asaeda;Qingyue Wang;Y. Kaneko;M. Rashid
中科院分区：
文献类型：
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作者：
Animesh Sarkar;T. Asaeda;Qingyue Wang;Y. Kaneko;M. Rashid
摘要：以盆栽形式研究了不同 Pb 浓度下丛枝菌根真菌 (AMF) 群落对芒草生长、生存能力、养分和铅吸收的影响。处理包括接种或不接种 AMF、Gigaspora margarita，以及向土壤中添加三种浓度的铅（0、100 和 1000 mg kg−1）。 Pb的添加显着减少了菌根定植。接种Pb的AMF增加了叶绿素含量、Fv/Fm、总干质量、吲哚-3-乙酸(IAA)、总氮和总磷，而与未接种处理相比，H2O2水平、吲哚-3-乙酸氧化酶(IAAO)活性和过氧化物酶(POD)活性较低。此外，AMF与Pb剂量一起施用会引起植物中Pb的浓度，其中较高剂量的Pb（1000 mg kg−1）导致植物地上部分的Pb含量较低，但根部的Pb含量较高。 G. margarita增强了M. sacchariflorus对Pb毒性的耐受性，促进了Pb在植物根部的积累，而最高剂量Pb（1000 mg kg−1）则抑制了向地上部的转运。然而，在污染土壤中，AMF对糖花的Pb去除能力非常显着。
ABSTRACT The effects of an arbuscular mycorrhizal fungi (AMF) association on the growth, survival capabilities, nutrients and lead (Pb) uptake of Miscanthus sacchariflorus under different Pb concentrations were studied in the form of pot cultures. The treatments comprised inoculation or non-inoculation of the AMF, Gigaspora margarita, and the addition of three Pb concentrations to the soil (0, 100 and 1000 mg kg−1). The addition of Pb significantly decreased mycorrhizal colonisation. The inoculation of AMF with Pb increased chlorophyll content, Fv/Fm, total dry mass, indole-3-acetic acid (IAA), total nitrogen, and total phosphorus, whereas H2O2 level, indole-3-acetic acid oxidase (IAAO) activity, and peroxidase (POD) activity were low compared to those in the non-inoculated treatments. Moreover, the application of AMF together with Pb doses induces concentrations of Pb in the plant, where the higher dose of Pb (1000 mg kg−1) induces a lower content of Pb in the aerial part of the plant but a higher content in the root. G. margarita enhanced the tolerance of M. sacchariflorus against Pb toxicity, and facilitated the accumulation of Pb in the plant roots, whereas translocation to the shoots was inhibited at the highest dose Pb (1000 mg kg−1). However, in contaminated soil, the Pb removal capability of M. sacchariflorus with AMF was remarkable.