Study of the rice straw biodegradation in mixed culture of Trichoderma viride and Aspergillus niger by GC-MS and FTIR
Study of the rice straw biodegradation in mixed culture of Trichoderma viride and Aspergillus niger by GC-MS and FTIR
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DOI：
10.1007/s11356-015-4149-8
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发表时间：
2015-07-01
影响因子：
5.8
通讯作者：
Zhou, Wei
中科院分区：
文献类型：
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作者：
Chen, Yaoning;Huang, Jingxia;Zhou, Wei
本研究采用气相色谱-质谱联用技术（GC-MS）和傅里叶变换红外光谱（FTIR）对稻秆提取的有机物和木质纤维素结构进行了研究，探讨了绿色木霉和黑曲霉混合培养的生物降解能力。气相色谱-质谱分析结果表明，与纯培养相比，混合培养在培养结束时具有较短的烷烃（庚烷）和较多的有机物（除烷烃外，共检测到29种有机物）。可以推断，有机质可以在一定程度上指示木质纤维素的降解程度。在混合培养的第5天和第10天，还检测到蒎烯，这可能代表了绿霉与黑霉的拮抗关系。FTIR光谱分析间接验证了气相色谱-质谱分析结果，表明混合培养比纯培养具有更好的秸秆降解效果。因此，本研究采用的方法可作为研究混合培养中木质纤维素降解机理的有效方法。
This study was conducted to investigate the biodegradation ability of the mixed culture of Trichoderma viride and Aspergillus niger through the study of the organic matter extracted from rice straw and the lignocellulose structure by using gas chromatography-mass spectrometer (GC-MS) and Fourier transforminfrared spectroscopy (FTIR). The results of the GC-MS showed that the mixed culture possessed shorter alkane (heptane) at the end of the incubation and more kinds of organic matter (except the alkanes, 29 kinds of organic matter were detected) than the pure cultures. It could be deduced that the organic matter could indicate the degradation degree of the lignocellulose to some extent. Moreover, pinene was detected in the mixed culture on days 5 and 10, which might represent the antagonistic relationship between T. viride and A. niger. The analysis of FTIR spectrums which indirectly verified the GC-MS results showed that the mixed culture possessed a better degradation of rice straw compared with the pure culture. Therefore, the methods used in this research could be considered as effective ones to investigate the lignocellulose degradation mechanism in mixed culture.