Graphite-like g-C3N4-F127-Au nanosheets used for sensitive monitoring of heat shock protein 90
Graphite-like g-C3N4-F127-Au nanosheets used for sensitive monitoring of heat shock protein 90
复制标题
类石墨 g-C3N4-F127-Au 纳米片用于热休克蛋白 90 的灵敏监测
DOI：
10.1016/j.snb.2017.10.065
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发表时间：
2018-03-01
影响因子：
8.4
通讯作者：
Liu, Fang
中科院分区：
文献类型：
--
作者：
Sun, Chong;Zhang, Muhan;Liu, Fang
本文构建了一种基于类石墨相氮化碳纳米片（g - C3N4 NSs）与金 - F127纳米粒子（g - C3N4 - F127 - Au NSs）杂化修饰电极的新型免疫传感器，用于热休克蛋白90（HSP90）的定量检测。利用透射电子显微镜（TEM）观察g - C3N4 - F127 - Au NSs的结构和形貌。通过紫外 - 可见光谱、傅里叶变换红外光谱（FTIR）和拉曼光谱对g - C3N4 - F127 - Au NSs的成功制备进行了表征。同时，通过线性扫描伏安法（LSV）和差分脉冲伏安法（DPV）测量其电化学性能。在优化条件下，峰电流与HSP90浓度在3.5μg/mL至2.43mg/mL范围内呈线性关系。线性回归方程为I（μA）= 10.31 - 10.05logc（R² = 0.9986，n = 9），检测限为2.67μg/mL。最后，该免疫传感器表现出良好的选择性，为肉类品质评估提供了一种新方法。（C）2017爱思唯尔有限公司。保留所有权利。
In this paper, a novel immunosensor was built for quantitative detection of heat shock protein 90 (HSP90) based on graphite-like carbon nitride nanosheets (g-C3N4 NSs) hybridized with gold-F127 nanoparticles (g-C3N4-F127-Au NSs) modified electrode. The transmission electron microscope (TEM) was used to observe the structure and morphology of g-C3N4-F127-Au NSs. The successful preparation of g-C3N4-F127-Au NSs was characterized by UV-vis spectra, fourier transform infrared spectra (FTIR) and Raman spectra. Meanwhile, the electrochemical properties were measured by linear sweep voltammetric (LSV) and differential pulse voltammetry (DPV). Under the optimized conditions, the peak currents were linear with the concentration of HSP90 ranging from 3.5 mu g/mL to 2.43 mg/mL. The linear regression equation was I (mu A) = 10.31-10.05logc (R-2 = 0.9986, n = 9) with the detection limit of 2.67 mu g/mL. Finally, the immunosensor exhibited good selectivity performance and provided a new approach for meat quality evaluation. (C) 2017 Elsevier B.V. All rights reserved.