Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis
Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis
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DOI：
10.1016/j.bmc.2010.12.001
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发表时间：
2011-02-01
影响因子：
3.5
通讯作者：
Mallet, Jean-Maurice
中科院分区：
文献类型：
--
作者：
Collot, Mayeul;Wilson, Iain B. H.;Mallet, Jean-Maurice
利用甲基叔丁基苯基（MBP）硫苷供体合成了植物和无脊椎动物n -聚糖的四种生物素化三糖和四糖片段，以评估它们作为交叉反应性碳水化合物决定因子（CCDs）参与交叉过敏。当结合物包被在链霉亲和素微孔板上时，观察到对抗蜜蜂抗体和抗hrp抗体的不同程度的反应性以及对过敏患者血清的反应性。结果表明，这些木基化和聚焦的低聚糖片段在确定CCD抗体表位方面具有潜在的应用价值。(C) 2010 Elsevier Ltd.版权所有。
Four biotinylated tri and tetrasaccharide fragments of plant and invertebrate N-glycans were synthesized using methyl tert-butyl phenyl (MBP) thioglycosides donors in order to evaluate their involvement in cross-allergies as cross-reactive carbohydrate determinants (CCDs). Various levels of reactivity to anti-bee and anti-HRP antibodies and with sera from allergic patients were observed when the conjugates were coated on streptavidin microplates. The results showed the potential utility of these xylosylated and fucosylated oligosaccharide fragments in determining CCD antibody epitopes. (C) 2010 Elsevier Ltd. All rights reserved.