Molecular typing of Japanese strains of Ralstonia solanacearum in relation to the ability to induce a hypersensitive reaction in tobacco
Molecular typing of Japanese strains of Ralstonia solanacearum in relation to the ability to induce a hypersensitive reaction in tobacco
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DOI：
10.1007/s10327-009-0188-7
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发表时间：
2009-10-01
影响因子：
1.2
通讯作者：
Ohnishi, Kouhei
中科院分区：
文献类型：
--
作者：
Liu, Yingqin;Kanda, Ayami;Ohnishi, Kouhei
根据烟草叶片过敏性反应(HR)和内切葡聚糖酶基因eg1、hrpB和gyrB的系统发育分析，对从日本各地不同寄主植物上分离的120个青枯雷尔氏菌菌株的遗传多样性进行了评价。EGL的系统发育分析表明，只有3株菌株属于系统IV型，117株属于系统I型，HrpB基因的部分序列在I型菌株中除1株外均相同。利用gyrB和egl基因片段的部分核苷酸序列将I型菌株分为11个gyrB型和8个eGL型，而利用GyrB和Egl的部分氨基酸序列将I型菌株分别分为4个GyrB型和5个EgI型。利用GyrB和Egl的多位点序列分型，我们在日本I型毒株中鉴定出10种独特的序列类型。GyrB42或GyrB66型菌株引起烟草青枯病，而GyrB2或GyrB9型菌株诱导烟草产生HR，说明烟草HR诱导在青枯病菌日本菌株中存在遗传分化。
The genetic diversity of 120 Ralstonia solanacearum strains isolated from a variety of host plants across Japan was assessed on the basis of hypersensitive response (HR) in tobacco leaves and phylogenetic analyses of endoglucanase gene egl, hrpB, and gyrB. Phylogenetic analysis of egl revealed that only three strains belonged to phylotype IV, and 117 strains belonged to phylotype I. Partial sequences of HrpB were identical among phylotype I strains except for one strain. Analyses using the partial nucleotide sequences of the gyrB and egl gene fragments grouped phylotype I strains into 11 gyrB and 8 egl types, respectively, whereas analyses using the partial amino acid sequences of GyrB and Egl grouped phylotype I strains into 4 GyrB and 5 Egl types, respectively. Using multilocus sequence typing of GyrB and Egl, we identified 10 unique sequence types within the Japanese phylotype I strains. Strains belonging to the GyrB42 or GyrB66 type caused wilt in tobacco, and strains belonging to GyrB2 or GyrB9 type elicited HR, demonstrating that HR induction in tobacco is genetically differentiated in the Japanese strains of R. solanacearum.