Silica nano-channel induced i-motif formation and stabilization at neutral and alkaline pH.
Silica nano-channel induced i-motif formation and stabilization at neutral and alkaline pH.
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DOI：
10.1039/c8cc02811g
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发表时间：
2018-06
影响因子：
4.9
通讯作者：
Sagar Satpathi;Konoya Das;P. Hazra
中科院分区：
文献类型：
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作者：
Sagar Satpathi;Konoya Das;P. Hazra
在这里，我们开发了一种新的策略，通过将富含C的序列纳入二氧化硅纳米通道内，在中性和碱性介质中稳定i基序DNA。随后，使用带正电荷的蛋白质实现了这种构象转变的可逆性。重要的是，这种整个构象转变可以在多个循环中进行，这提供了除pH和热退火之外的控制i基序形成的替代方式。
Here, we have developed a new strategy to stabilize i-motif DNA in neutral and alkaline media by incorporating C-rich sequences inside silica nano-channels. Subsequently, the reversibility of this conformational transition has been achieved using a positively charged protein. Importantly, this entire conformational transition can be performed in multiple cycles, which offers an alternative way to control i-motif formation other than pH and thermal annealing.