The constitutive expression of Chrysanthemum dichrum ICE1 in Chrysanthemum grandiflorum improves the level of low temperature, salinity and drought tolerance
The constitutive expression of Chrysanthemum dichrum ICE1 in Chrysanthemum grandiflorum improves the level of low temperature, salinity and drought tolerance
复制标题
菊花ICE1在菊花中的组成型表达提高了菊花的耐低温、耐盐性和耐旱水平
DOI：
10.1007/s00299-012-1288-y
复制
发表时间：
2012-09-01
影响因子：
6.2
通讯作者：
Fang, Weimin
中科院分区：
文献类型：
--
作者：
Chen, Lin;Chen, Yu;Fang, Weimin
菊花的品质和产量受到各种非生物胁迫的严重影响。本研究利用RACE PCR技术从菊花中分离到CdICE 1基因，该基因与菊花品种'金霸'的ICE 1 ORF具有相同的核苷酸序列。CdICE 1含有一个保守的bHLH结构域、一个核定位结构域、一个富S基序和一个ACT结构域。CdICE 1在C. grandiflorum提高了C. grandiflorum对低温/冰冻、干旱和盐碱的适应性。当转基因以反义方向插入时，内源ICE 1基因的表达下调，并且植物对非生物胁迫的敏感性水平增加。在逆境胁迫下，正义转基因植株CgDREBa和CgDREBb的表达水平、超氧化物歧化酶和过氧化物酶活性以及脯氨酸含量均升高，而反义转基因植株CgDREBa和CgDREBb的表达水平、超氧化物歧化酶和过氧化物酶活性以及脯氨酸含量均降低。总之，CdICE 1代表了一个有希望的候选人的生物技术方法，以提高作物的非生物胁迫耐受性的水平。洋桔梗通过调节参与氧化和渗透稳态途径的CgDREB来赋予胁迫耐受性。
The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved bHLH domain, a nuclear localization domain, a S-rich motif and a ACT domain. The constitutive expression of CdICE1 in C. grandiflorum improved the tolerance of C. grandiflorum to low temperature/freezing, drought and salinity. When the transgene was inserted in the antisense direction, the expression of the endogenous ICE1 gene was down-regulated, and the level of the plant's sensitivity to abiotic stress increased. The level of expression of CgDREBa and CgDREBb, activities of superoxide dismutase and peroxidase and the proline content were enhanced in the sense transgenic lines, and lowered in the antisense ones under stresses. In conclusion, CdICE1 represents a promising candidate for a biotechnological approach to improve the level of crop abiotic stress tolerance.Key message:Overexpression of CdICE1 in C. grandiflorum confers the stress tolerance via its regulation of CgDREB involved in the oxidative and osmotic homeostasis pathways.