HUMAN AMYLOID PRECURSOR PROTEIN AMELIORATES BEHAVIORAL DEFICIT OF FLIES DELETED FOR APPL GENE
HUMAN AMYLOID PRECURSOR PROTEIN AMELIORATES BEHAVIORAL DEFICIT OF FLIES DELETED FOR APPL GENE
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DOI：
10.1016/0896-6273(92)90024-8
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发表时间：
1992-10-01
期刊：
影响因子：
16.2
通讯作者：
WHITE, K
中科院分区：
文献类型：
--
作者：
LUO, LQ;TULLY, T;WHITE, K
果蝇淀粉样前体蛋白样（Appl）基因编码与阿尔茨海默病相关的β -淀粉样前体蛋白（APP）相似的蛋白产物（Appl）。为了了解APPL蛋白在体内的功能，我们制作了苹果基因缺失的果蝇。这些苍蝇是可存活的、可繁殖的、形态正常的，但它们表现出微妙的行为缺陷。我们发现，通过表达野生型（而不是突变型）Appl蛋白的转基因，苹果蝇的快速趋光性缺陷得到了部分修复。我们进一步证明了APPL和APP之间的功能同源性，因为表达人类APP的转基因与表达苍蝇APPL的转基因表现出相似的拯救水平。
Drosophila amyloid precursor protein-like (Appl) gene encodes a protein product (APPL) similar to beta-amyloid precursor protein (APP) associated with Alzheimer's disease. To understand the in vivo function of APPL protein, we have generated flies deleted for the Appl gene. These flies are viable, fertile, and morphologically normal, yet they exhibit subtle behavioral deficits. We show that a fast phototaxis defect in Appl- flies is partially rescued by transgenes expressing the wild-type, but not a mutant, APPL protein. We further demonstrate a functional homology between APPL and APP, since transgenes expressing human APP show a similar level of rescue as transgenes expressing fly APPL.