Anti-inflammatory and anti-osteoarthritis effects of fermented Achyranthes japonica Nakai
Anti-inflammatory and anti-osteoarthritis effects of fermented Achyranthes japonica Nakai
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DOI：
10.1016/j.jep.2012.05.020
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发表时间：
2012-08-01
影响因子：
5.4
通讯作者：
Choi, Sang-Won
中科院分区：
文献类型：
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作者：
Lee, Sung-Gyu;Lee, Eun-Ju;Choi, Sang-Won
民族药理学相关性：日本牛膝（AJN）传统上用于控制疼痛和改善骨关节炎（OA）患者的功能障碍。研究目的：本研究的目的是研究发酵AJN（FAJN）的抗炎和抗骨关节炎活性。材料与方法：采用LPS诱导的RAW 264.7细胞体外实验评价非发酵AJN（NFAJN）和FAJN的抗炎活性。此外，还通过体外SW 1353细胞实验和体内胶原酶诱导的兔关节炎（CIA）模型实验，观察了它们对软骨的保护作用。结果：FAJN通过抑制LPS诱导的RAW 264.7细胞NO和PGE 2的产生，以及抑制TNF-α诱导的SW 1353细胞MMP-3的释放，表现出较强的抗炎活性。FAJN含有较高水平的20-羟乙基淀粉，作为一种标志物成分，比AJN。FAJN通过抑制局部炎症来改善OA的进展。它通过调节TNF-α和IL-4的水平，并保护关节软骨，防止破坏蛋白多糖，胶原蛋白，并防止损伤软骨cytos.Conclusion：因此，FAJN是一个潜在的治疗剂，减少软骨损伤，发生在OA。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Achyranthyes japonica Nakai (AJN) has been traditionally used to control pain and improve dysfunction in osteoarthritis (OA) patients. Aim of the study: The objectives of the present study were to investigate anti-inflammatory and anti-osteoarthritis activities of fermented AJN (FAJN).Materials and methods: Anti-inflammatory activity of non-fermented AJN (NFAJN) and FAJN was evaluated by in vitro assay using LPS-induced RAW 264.7 cells. In addition, their cartilage protective effects were also determined in vitro assay using SW1353 cell and in vivo model system using collagenase-induced arthritis (CIA) in rabbits. Moreover, we isolated and identified 20-hydroxyecdysone (20-HES) as a marker component in FAJN.Result: FAJN showed stronger anti-inflammatory activity than NFAJN through inhibiting production of NO and PGE2 in LPS-induced RAW 264.7, and lowering levels of MMP-3 release in SW1353 cells treated with TNF-a. FAJN contained higher levels of 20-HES, as a marker component, than AJN. FAJN ameliorates the progress of OA by inhibiting local inflammation. It does this by regulating levels of TNF-a and IL-4, and protecting articular cartilage by preventing destruction of proteoglycan, collagens, and also preventing injury to chondrocytes.Conclusion: Therefore, FAJN is a potential therapeutic agent for reduction of cartilage damage that occurs in OA. (C) 2012 Elsevier Ireland Ltd. All rights reserved.