Abnormal angiogenesis of placenta in progranulin-deficient mice
Abnormal angiogenesis of placenta in progranulin-deficient mice
复制标题
DOI：
10.3892/mmr.2020.11438
复制
发表时间：
2020-08
影响因子：
3.4
通讯作者：
Bai-Ruo Xu;Xingyou Chen;Yubin Ding;Chang Chen;Taihang Liu;Hua Zhang
中科院分区：
文献类型：
--
作者：
Bai-Ruo Xu;Xingyou Chen;Yubin Ding;Chang Chen;Taihang Liu;Hua Zhang
Progranulin (PGRN) is a secreted growth factor involved in pleiotropic functions, particularly angiogenesis. A distinctly different placental expression of PGRN has been reported between normal pregnancies and pregnancies with complications, such as pre-eclampsia or fetal growth restriction. However, the role of PGRN in placental vascular development remains to be elucidated. In the present study, PGRN-knockout mice (PGRN−/−) were used to investigate the role of PGRN in the development of placental blood vessels and placental formation. Placental weights and pup body weights were significantly lower in the PGRN−/− mice compared with the wild-type mice. Reduced labyrinthine layer areas and aberrant vascularization were also observed via hematoxylin and eosin staining of PGRN−/− mice at embryonic day 14.5 (E14.5) and E17.5. In addition, the morphological data obtained via immunohistochemistry, immunofluorescence staining and western blotting demonstrated decreased expression levels of the blood vessel markers α-smooth muscle actin and CD31 in PGRN−/− placentas. Furthermore, vasodilator endothelial nitric oxide synthase was reduced in the PGRN−/− placenta. These results indicated that PGRN serves an essential role in the normal angiogenesis of the placental labyrinth in mice.
