Changes in Chemical Contents and Enzyme Activities during Embryonic Development of Bluefin Tuna
Changes in Chemical Contents and Enzyme Activities during Embryonic Development of Bluefin Tuna
复制标题
蓝鳍金枪鱼胚胎发育过程中化学成分和酶活性的变化
DOI：
10.2331/fishsci.63.1014
复制
发表时间：
1997
影响因子：
1.9
通讯作者：
H. Kumai
中科院分区：
文献类型：
--
作者：
K. Takii;S. Miyashita;M. Seoka;Yuji Tanaka;Y. Kubo;H. Kumai
蛋白质含量随发育进程逐渐增加，游离氨基酸含量逐渐减少。三酰甘油（TG）是卵裂早期420 mg/g干卵的主要成分，到原肠胚晚期后迅速下降，到出壳前达到140 mg/g干卵。谷草转氨酶、谷丙转氨酶、肌酸激酶和乳酸脱氢酶的活性在卵裂早期和枯否氏泡之间下降，之后上升。碱性磷酸酶活性在胚胎形成前一直保持较高水平，但此后迅速下降。这些结果表明，TG作为主要的能量燃料起着重要的作用，并且在器官的分化和形成中起着重要的作用。
protein gradually increased, but free amino acid decreased with the development. Triacylglycerole (TG), a main egg constituent of 420 mg/g dry egg at the stage of early cleavage, rapidly decreased after the stage of late gastrula and reached 140 mg/g dry egg justbefore hatching. Activities of aspartate aminotransferase, alanine aminotransferase, creatine kinase, and lactate dehydrogenase fellbetween the stages of early cleavage and Kupffer's vesicle and rose thereafter. Activities of alkaline phosphatase remained high until embryo formation but fell rapidly thereafter. These results indicate that TG plays an important role as a main energy fuel, and that the differentiation and formation of organs are