Characterization of solubilized bradykinin B2 receptors from smooth muscle and mucosa of guinea pig ileum.
Characterization of solubilized bradykinin B2 receptors from smooth muscle and mucosa of guinea pig ileum.
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豚鼠回肠平滑肌和粘膜溶解的缓激肽 B2 受体的表征。
DOI：
10.1016/0006-2952(92)90716-v
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发表时间：
1992
影响因子：
5.8
通讯作者：
C. Goodman
中科院分区：
文献类型：
--
作者：
R. Ransom;G. S. Young;K. Schneck;C. Goodman
豚鼠回肠缓激素b2受体以膜和可溶性两种形式存在。[3H]BK在回肠纵肌、圆肌和粘膜层制备的膜上结合在一类几乎相同亲和力的位点上。在不同的层结合的药理学是难以区分的。洗涤剂3-[（3-胆酰胺丙基）-二甲酰胺]-1-丙烷磺酸盐（CHAPS）以非常稳定的构象最大限度地溶解了近80%的膜结合活性。在可溶性制剂中，[3H]BK标记了一类位点，但亲和力降低了约10倍。在竞争研究中，BK类似物的亲和力也同样降低。从回肠不同层制备的可溶性受体的结合在药理学上没有差异。结果表明，回肠是可溶性b2受体的良好来源，平滑肌和粘膜中的受体非常相似。
Bradykinin (BK) B2receptors in guinea pig ileum were characterized in both membrane and soluble form. [3H]BK bound to a single class of sites with almost identical affinities in membranes prepared from the longitudinal muscle, circular muscle and mucosal layers of the ileum. The pharmacology of the binding in the distinct layers was indistinguishable. The detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS) maximally solubilized nearly 80% of membrane binding activity in a very stable conformation. In soluble preparations, [3H]BK labeled a single class of sites but with about 10-fold lower affinity. The affinities of BK analogs in competition studies were similarly reduced. There was no difference in the pharmacology of the binding in soluble receptors prepared from the different layers of the ileum. The results show that the ileum is a good source of solubilized B2receptors and that the receptors in the smooth muscle and the mucosa are very similar.
两个具有皮摩尔亲和力的缓激肽结合位点。
DOI：
--
发表时间：
1986
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Manning,DC;Vavrek,R;Stewart,JM;Snyder,SH
通讯作者：
Snyder,SH
DOI：
10.1073/pnas.88.17.7724
发表时间：
1991-09-01
影响因子：
11.1
作者：
MCEACHERN, AE;SHELTON, ER;JARNAGIN, K
通讯作者：
JARNAGIN, K