Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold
Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold
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DOI：
10.1302/2046-3758.82.bjr-2018-0134.r1
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发表时间：
2019-02-01
影响因子：
4.6
通讯作者：
Grigolo, B.
中科院分区：
文献类型：
--
作者：
Filardo, G.;Petretta, M.;Grigolo, B.
目的：半月板损伤常与积极的生活方式有关。半月板组织的损伤使年轻患者接受半月板手术的风险更高，因此患骨关节炎的风险也更高。在这项研究中，我们进行了概念验证研究，利用3D生物打印技术开发细胞化的人体半月板。方法基于人类志愿者的MRI扫描，建立生物工程半月板内侧组织的三维模型。使用专用软件处理来自这些MRI扫描的数字成像和医学通信（DICOM）数据，以获得该结构的STL模型。所选择的3D Discovery打印工具是一种基于微阀的喷墨打印头。从骨髓中分离出原代间充质干细胞（MSCs），并在打印前将其包埋在胶原基生物墨水中。对携带MSCs的实现细胞负载构建体进行LIVE/DEAD实验，以评估细胞分布和活力。结果本研究采用3D生物打印技术实现了人体细胞负载胶原半月板。半月板原型显示了该技术的生物学潜力，可以在生物相容性材料上提供具有活细胞的解剖形状、患者特异性结构。结论本文报道了基于胶原蛋白的人体半月板的初步研究结果。所描述的原型可以作为这种基于胶原蛋白的组织工程结构的未来发展的起点，它可以帮助优化设计用于替代受损半月板的植入物。
ObjectivesMeniscal injuries are often associated with an active lifestyle. The damage of meniscal tissue puts young patients at higher risk of undergoing meniscal surgery and, therefore, at higher risk of osteoarthritis. In this study, we undertook proof-of-concept research to develop a cellularized human meniscus by using 3D bioprinting technology.MethodsA 3D model of bioengineered medial meniscus tissue was created, based on MRI scans of a human volunteer. The Digital Imaging and Communications in Medicine (DICOM) data from these MRI scans were processed using dedicated software, in order to obtain an STL model of the structure. The chosen 3D Discovery printing tool was a microvalve-based inkjet print-head. Primary mesenchymal stem cells (MSCs) were isolated from bone marrow and embedded in a collagen-based bio-ink before printing. LIVE/DEAD assay was performed on realized cell-laden constructs carrying MSCs in order to evaluate cell distribution and viability.ResultsThis study involved the realization of a human cell-laden collagen meniscus using 3D bioprinting. The meniscus prototype showed the biological potential of this technology to provide an anatomically shaped, patient-specific construct with viable cells on a biocompatible material.ConclusionThis paper reports the preliminary findings of the production of a custom-made, cell-laden, collagen-based human meniscus. The prototype described could act as the starting point for future developments of this collagen-based, tissue-engineered structure, which could aid the optimization of implants designed to replace damaged menisci.