Effect of human plasma-type platelet-activating factor acetylhydrolase in two anaphylactic shock models
Effect of human plasma-type platelet-activating factor acetylhydrolase in two anaphylactic shock models
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DOI：
10.1016/s0014-2999(99)00920-6
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发表时间：
2000-02-25
影响因子：
5
通讯作者：
Nakanishi, T
中科院分区：
文献类型：
--
作者：
Fukuda, Y;Kawashima, H;Nakanishi, T
在PAF致小鼠死亡模型和活动性过敏模型中检测了人重组血浆型血小板活化因子（PAF）乙酰水解酶的作用。在PAF诱导的死亡模型中，小鼠静脉注射40 μ g/kg PAF， PAF乙酰水解酶以剂量依赖的方式降低死亡率，1.0 mg/kg PAF乙酰水解酶完全预防死亡。注射PAF后，肺中性粒细胞标志物髓过氧化物酶活性升高，PAF乙酰水解酶处理显著逆转髓过氧化物酶活性升高。与PAF诱导的模型一样，PAF乙酰水解酶也降低了活性过敏性休克模型的死亡率，在活性过敏性休克模型中，静脉注射牛血清白蛋白给预先接种牛血清白蛋白的小鼠。1.0 mg/kg PAF乙酰水解酶对大鼠死亡率的保护作用显著。这些结果表明，PAF是全身性过敏反应致死率的重要介质，并且PAF乙酰水解酶可能有益于治疗过敏性休克(C) 2000 Elsevier Science B.V.。
The effect of human recombinant plasma-type platelet-activating factor (PAF) acetylhydrolase was examined in two murine models, PAF-induced death and active anaphylactic models. In the PAF-induced death model where mice were injected intravenously with 40 mu g/kg of PAF, the administration of PAF acetylhydrolase reduced mortality in a dose-dependent manner, showing complete prevention of mortality at 1.0 mg/kg. Myeloperoxidase activity, the marker for neutrophils, was increased in the lung by PAF injection, and the PAF acetylhydrolase treatment significantly reversed the increase in myeloperoxidase activity. As in the PAF-induced model, PAF acetylhydrolase also decreased mortality in the active anaphylactic shock model where bovine serum albumin was injected intravenously to mice previously immunized with bovine serum albumin. The protective effect of PAF acetylhydrolase on mortality in this model was significant at 1.0 mg/kg. These results suggest that PAF is an important mediator in the lethality of systemic anaphylaxis, and that PAF acetylhydrolase may be beneficial for treatment of anaphylactic shock (C) 2000 Elsevier Science B.V. All rights reserved.