Impact of Physical Inactivity on Subcutaneous Adipose Tissue Metabolism in Healthy Young Male Offspring of Patients With Type 2 Diabetes
Impact of Physical Inactivity on Subcutaneous Adipose Tissue Metabolism in Healthy Young Male Offspring of Patients With Type 2 Diabetes
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DOI：
10.2337/db10-0320
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发表时间：
2010-11-01
期刊：
影响因子：
7.7
通讯作者：
Stallknecht, Bente
中科院分区：
文献类型：
--
作者：
Hojbjerre, Lise;Sonne, Mette Paulli;Stallknecht, Bente
目的：缺乏运动是2型糖尿病的一个危险因素，在一级亲属（FDR）受试者中可能更有害，揭示了潜在的代谢缺陷。利用2型糖尿病阳性家族史作为遗传风险增加的标志，本研究的目的是调查运动不足对FDR受试者脂肪组织（AT）代谢的影响。研究设计与方法-共有13名FDR和20名对照（CON）受试者参与研究。所有患者在卧床休息10天之前和之后，使用葡萄糖钳技术结合微透析技术测量葡萄糖摄取、脂肪分解和皮下腹部（SCAAT）和股脂肪组织（SCFAT）的乳酸释放。此外，在SCAAT活检中检测脂肪酶的mRNA表达。结果：在睡眠休息前，FDR受试者在scat中葡萄糖摄取显著增加。此外，脂肪酶mRNA的表达在FDR受试者的scat中显著降低。卧床休息显著减少脂肪分解，并倾向于增加CON和FDR受试者的SCFAT中的葡萄糖摄取。作为卧床休息的反应，CON受试者的SCAAT葡萄糖摄取显著增加，而FDR受试者则没有。结论- fdr受试者表现出异常的AT代谢，包括卧床休息前葡萄糖摄取增加。然而，在床上休息时，FDR和CON受试者在AT代谢方面的差异被减弱，因为CON受试者相对于FDR受试者有更多的不利变化。就AT代谢紊乱而言，运动不足本身对FDR受试者的危害并不比CON受试者大。糖尿病杂志，2010
OBJECTIVE-Physical inactivity is a risk factor for type 2 diabetes and may be more detrimental in first-degree relative (FDR) subjects, unmasking underlying defects of metabolism. Using a positive family history of type 2 diabetes as a marker of increased genetic risk, the aim of this study was to investigate the impact of physical inactivity on adipose tissue (AT) metabolism in FDR subjects.RESEARCH DESIGN AND METHODS-A total of 13 FDR and 20 control (CON) subjects participated in the study. All were studied before and after 10 days of bed rest using the glucose clamp technique combined with measurements of glucose uptake, lipolysis, and lactate release from subcutaneous abdominal (SCAAT) and femoral (SCFAT) adipose tissue by the microdialysis technique. Additionally, mRNA expression of lipases was determined in biopsies from SCAAT.RESULTS-Before bed rest, the FDR subjects revealed significantly increased glucose uptake in SCAAT. Furthermore, mRNA expression of lipases was significantly decreased in the SCAAT of FDR subjects. Bed rest significantly decreased lipolysis and tended to increase glucose uptake in the SCFAT of both CON and FDR subjects. In response to bed rest, SCAAT glucose uptake significantly increased in CON subjects but not in FDR subjects.CONCLUSIONS-FDR subjects exhibit an abnormal AT metabolism including increased glucose uptake prior to bed rest. However, the differences between FDR and CON subjects in AT metabolism were attenuated during bed rest due to relatively more adverse changes in CON subjects compared with FDR subjects. Physical inactivity per se is not more deleterious in FDR subjects as compared with CON subjects with respect to derangements in AT metabolism. Diabetes 59:2790-2798, 2010