Quercetin, an inhibitor of lactate transport and a hyperthermic sensitizer of HeLa cells.
Quercetin, an inhibitor of lactate transport and a hyperthermic sensitizer of HeLa cells.
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DOI：
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发表时间：
1984
期刊：
影响因子：
11.2
通讯作者：
J. Kim;S. Kim;A. Alfieri;C. Young
中科院分区：
文献类型：
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作者：
J. Kim;S. Kim;A. Alfieri;C. Young
由于癌细胞产生大量的乳酸通过有氧糖酵解，因为酸性pH值已被证明有选择性地增强热疗的细胞毒性作用，我们正在研究细胞暴露于药物，抑制乳酸转运和降低细胞内pH值后的热疗的细胞毒性作用的影响。槲皮素是一种产生乳酸转运抑制作用的生物素，在37度（0.1 mM）下长达4小时没有细胞毒性。当HeLa细胞在41和42度暴露于槲皮素时，观察到高血压诱导的细胞毒性的显著增强。增强的幅度取决于药物浓度、培养基的pH值、温度和治疗持续时间。与此相反，用芦丁（一种结构上相关的生物素，缺乏乳酸转运抑制作用）处理细胞，没有显示出热增强作用。
Since cancer cells produce large amounts of lactate via aerobic glycolysis and since an acidic pH has been shown to selectively enhance the cytotoxic effects of hyperthermia, we are examining the influence of cell exposure to drugs which inhibit lactate transport and lower intracellular pH upon cytotoxic effects of hyperthermia. Quercetin, a bioflavonoid that produces lactate transport inhibition, was not cytotoxic up to 4 hr at 37 degrees (0.1 mM). When HeLa cells were exposed to quercetin at 41 and 42 degrees, significant potentiation of hyperthermia-induced cytotoxicity was observed. The magnitude of the potentiation was dependent on the drug concentration, pH of the culture medium, temperature, and duration of treatment. In contrast, treatment of cells with rutin, a structurally related bioflavonoid that lacks the property of lactate transport inhibition, showed no hyperthermic potentiation.