Pathfinding during spinal tract formation in the chick-quail chimera analysed by species-specific monoclonal antibodies.
Pathfinding during spinal tract formation in the chick-quail chimera analysed by species-specific monoclonal antibodies.
复制标题
通过物种特异性单克隆抗体分析鸡-鹌鹑嵌合体脊髓束形成过程中的寻路。
作者：
H. Tanaka;M. Kinutani;A. Agata;Y. Takashima;K. Obata
为了研究鸡胚发育早期脊髓束的形成，制备了鸡-鹌鹑脊髓嵌合体，并制备了鸡-鹌鹑脊髓嵌合体的种特异性单克隆抗体。MAb CN、QN和CQN分别独特地染色鸡、鹌鹑以及鸡和鹌鹑两者的神经组织。所有三种抗体似乎都与相同的膜分子结合，但与不同的表位结合。脊髓反转显示了脊髓中间神经元和背根神经节细胞的轴突生长特征。鹌鹑脊髓中间神经元沿着原腹侧边缘层生长在鹌鹑脊髓移植在一个颠倒的位置，然后转向腹侧之间的移植物和宿主之间的边界，并沿着宿主鸡腹侧边缘层生长。背根神经节的中央轴突仅限于脊髓腹外侧区，最初形成背索。这些结果表明，脊髓中间神经元和背根神经节细胞积极选择生长沿着特定区域的脊髓和脊髓束的形成似乎是由脊髓细胞，而不是由硬结细胞。
In order to analyse the spinal tract formation at early stages of development in avian embryos, chick-quail spinal cord chimeras were prepared and species-specific monoclonal antibodies (MAb) were developed. MAbs CN, QN and CQN uniquely stained chick, quail, and both chick and quail nervous tissues, respectively. All three antibodies appeared to bind to the same membrane molecule, but to different epitopes. Cord reversal revealed the features of axonal growth of both cord interneurons and dorsal root ganglion cells. Quail cord interneurons grew along an originally ventral marginal layer in the quail cord transplanted in a reversed position, then turned toward the ventral side at the boundary between the graft and the host, and grew along the host chick ventral marginal layer. Central axons of dorsal root ganglia were restricted to the ventrolateral region of the cord which originally formed the dorsal funiculus. These results suggest that cord interneurons and dorsal root ganglion cells actively select to grow along specific regions of the cord and that spinal tract formation appears to be determined by cord cells, and not by sclerotome cells.