Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia
Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia
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DOI：
10.1186/1743-422x-7-226
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发表时间：
2010-09-14
期刊：
影响因子：
4.8
通讯作者：
Gallego-Gomez, Juan C.
中科院分区：
文献类型：
--
作者：
Mendez, Jairo A.;Usme-Ciro, Jose A.;Gallego-Gomez, Juan C.
背景资料：登革热是最重要的病毒性疾病之一，影响全球约5000万人，特别是在热带和亚热带国家。在哥伦比亚，该病毒于70年代初首次被发现，当时该疾病成为主要的公共卫生问题。从那时起，所有四种血清型的病毒都有报告。结果：利用74株哥伦比亚分离株的E基因羧基端224 bp序列进行系统进化分析，并对时间差异进行估计，结果表明，哥伦比亚分离株的E基因羧基端224 bp序列与国外分离株的E基因羧基端224 bp序列同源性较高，与国外分离株的E基因羧基端224 bp序列同源性较高。分析的DENV-1哥伦比亚分离株属于以前定义的基因型V。只有一个病毒分离株被归类为基因型I，可能代表了没有传播的唯一引入。最古老的菌株与1977年在加勒比海首次在美国发现的菌株密切相关，并被发现了两年，直到大约六年后消失。1987年左右，分离产生了2个谱系，它们分别进化，但在分析区域没有发现主要的氨基酸变化。结论：DENV-1自1978年以来在哥伦比亚流行。然而，沿着涵盖的时间段分离的毒株之间的系统发育关系表明，在某些年份检测到的病毒毒株虽然属于相同的基因型V，但具有不同的近期来源，对应于在邻国传播的病毒毒株的多次重新引入事件。本研究中使用的病毒株没有形成单系群，这是多系起源的证据。我们报告了不同DENV-1谱系的快速传播模式和高进化率。
Background: Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70's when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV 1), there are not studies about its origin, genetic diversity and distribution.Results: We used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major aminoacid changes in the analyzed region were found.Conclusion: DENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages.